TY - JOUR
T1 - Degradation of synthetic fragrances by laccase-mediated system
AU - Vallecillos, Laura
AU - Sadef, Yumna
AU - Borrull, Francesc
AU - Pocurull, Eva
AU - Bester, Kai
PY - 2017/7/15
Y1 - 2017/7/15
N2 - Laccase mediator systems are important biodegradation agents as the rate of reaction could be enhanced in the presence of redox mediators. In the present study the commercial enzyme laccase from Trametes versicolor and the redox mediator 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were used for the biotransformation of the synthetic fragrances 1-(1,2,3,4,5,6,7,8-octahydro-2,3,8,8,-tetramethyl-2-naphthyl)ethan-1-one (Iso-E-Super, OTNE), 1,3,4,6,7,8,-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta-[g]-2-benzopyran (Galaxolide, HHCB), 7-acetyl-1,1,3,4,4,6-hexamethyl-1,2,3,4-tetrahydronaphtalene (Tonalide, AHTN) and the transformation product of HHCB, 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta-[g]-2-benzopyran-1-one (Galaxolidone, HHCB-lactone) in water. A particular focus was to assess the effects of the enzyme laccase from Trametes versicolor in the enantioselective degradation of the target compounds, for this reason gas chromatography with an enantioselective column was used as separation technique followed by mass spectrometry detection. In addition, as enantioselective degradation of musk fragrances was observed in wastewater, sewage sludge and fish samples, enantiomeric fractions of selected compounds were studied during composting. In a period of 144 h, the target fragrances could be effectively removed by the enzyme laccase with removal percentages greater than 70%, except AHTN with a removal percentage of 42%. However, the degradation process prompted by the enzyme laccase was shown to be non-enantioselective as no significant differences were observer between the enantiomeric fractions calculated at the beginning and at the end of the degradation process. Meanwhile, the composting process was shown to be enantioselective.
AB - Laccase mediator systems are important biodegradation agents as the rate of reaction could be enhanced in the presence of redox mediators. In the present study the commercial enzyme laccase from Trametes versicolor and the redox mediator 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were used for the biotransformation of the synthetic fragrances 1-(1,2,3,4,5,6,7,8-octahydro-2,3,8,8,-tetramethyl-2-naphthyl)ethan-1-one (Iso-E-Super, OTNE), 1,3,4,6,7,8,-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta-[g]-2-benzopyran (Galaxolide, HHCB), 7-acetyl-1,1,3,4,4,6-hexamethyl-1,2,3,4-tetrahydronaphtalene (Tonalide, AHTN) and the transformation product of HHCB, 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta-[g]-2-benzopyran-1-one (Galaxolidone, HHCB-lactone) in water. A particular focus was to assess the effects of the enzyme laccase from Trametes versicolor in the enantioselective degradation of the target compounds, for this reason gas chromatography with an enantioselective column was used as separation technique followed by mass spectrometry detection. In addition, as enantioselective degradation of musk fragrances was observed in wastewater, sewage sludge and fish samples, enantiomeric fractions of selected compounds were studied during composting. In a period of 144 h, the target fragrances could be effectively removed by the enzyme laccase with removal percentages greater than 70%, except AHTN with a removal percentage of 42%. However, the degradation process prompted by the enzyme laccase was shown to be non-enantioselective as no significant differences were observer between the enantiomeric fractions calculated at the beginning and at the end of the degradation process. Meanwhile, the composting process was shown to be enantioselective.
KW - Biotransformation
KW - Enantioselective chromatography
KW - Laccase
KW - Synthetic fragrances
UR - http://www.scopus.com/inward/record.url?scp=85017410118&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2017.04.003
DO - 10.1016/j.jhazmat.2017.04.003
M3 - Journal article
AN - SCOPUS:85017410118
SN - 0304-3894
VL - 334
SP - 233
EP - 243
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
ER -