TY - JOUR
T1 - Exposure of the Pig CNS for Histological Analysis
T2 - A Manual for Decapitation, Skull Opening, and Brain Removal
AU - Bjarkam, Carsten R
AU - Orlowski, Dariusz
AU - Tvilling, Laura
AU - Bech, Johannes
AU - Glud, Andreas Nørgaard
AU - Sørensen, Jens-Christian H
PY - 2017/4/13
Y1 - 2017/4/13
N2 - Pigs have become increasingly popular in large-animal translational neuroscience research as an economically and ethically feasible substitute to non-human primates. The large brain size of the pig allows the use of conventional clinical brain imagers and the direct use and testing of neurosurgical procedures and equipment from the human clinic. Further macroscopic and histological analysis, however, requires postmortem exposure of the pig central nervous system (CNS) and subsequent brain removal. This is not an easy task, as the pig CNS is encapsulated by a thick, bony skull and spinal column. The goal of this paper and instructional video is to describe how to expose and remove the postmortem pig brain and the pituitary gland in an intact state, suitable for subsequent macroscopic and histological analysis.
AB - Pigs have become increasingly popular in large-animal translational neuroscience research as an economically and ethically feasible substitute to non-human primates. The large brain size of the pig allows the use of conventional clinical brain imagers and the direct use and testing of neurosurgical procedures and equipment from the human clinic. Further macroscopic and histological analysis, however, requires postmortem exposure of the pig central nervous system (CNS) and subsequent brain removal. This is not an easy task, as the pig CNS is encapsulated by a thick, bony skull and spinal column. The goal of this paper and instructional video is to describe how to expose and remove the postmortem pig brain and the pituitary gland in an intact state, suitable for subsequent macroscopic and histological analysis.
KW - Journal Article
U2 - 10.3791/55511
DO - 10.3791/55511
M3 - Journal article
C2 - 28447999
SN - 1940-087X
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
IS - 122
M1 - 55511
ER -