Abstract
Production of bioactive compounds and enzymes from filamentous fungi is highly dependent
on cultivation conditions. Here we present an easy way to cultivate filamentous fungi on glass beads
which allow complete control of nutrient supply. Secondary metabolite production in Fusarium
graminearum and Fusarium solani cultivated on agar plates, in shaking liquid culture or on glass beads
was compared. Agar plate culture and glass bead cultivation yielded comparable results while liquid
culture had lower production of secondary metabolites. RNA extraction from glass beads and liquid
cultures was easier than from agar plates and the quality was superior. The system allows simple
control of nutrient availability throughout fungal cultivation. This combined with the ease of extraction
of nucleic acids and metabolites makes the system highly suitable for the study of gene regulation in
response to specific nutrient factors.
on cultivation conditions. Here we present an easy way to cultivate filamentous fungi on glass beads
which allow complete control of nutrient supply. Secondary metabolite production in Fusarium
graminearum and Fusarium solani cultivated on agar plates, in shaking liquid culture or on glass beads
was compared. Agar plate culture and glass bead cultivation yielded comparable results while liquid
culture had lower production of secondary metabolites. RNA extraction from glass beads and liquid
cultures was easier than from agar plates and the quality was superior. The system allows simple
control of nutrient availability throughout fungal cultivation. This combined with the ease of extraction
of nucleic acids and metabolites makes the system highly suitable for the study of gene regulation in
response to specific nutrient factors.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Journal of Microbiological Methods |
| Vol/bind | 94 |
| Udgave nummer | 3 |
| Sider (fra-til) | 343-346 |
| ISSN | 0167-7012 |
| DOI | |
| Status | Udgivet - 2013 |