TY - JOUR
T1 - Photonic modulation of epidermal growth factor receptor halts receptor activation and cancer cell migration
AU - Botelho, Cláudia M
AU - Gonçalves, Odete
AU - Marques, Rogério
AU - Thiagarajan, Viruthachalam
AU - Vorum, Henrik
AU - Gomes, Andreia C.
AU - Neves-Petersen, Maria Teresa
N1 - © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2018/9
Y1 - 2018/9
N2 - Epidermal growth factor receptor (EGFR) plays a key role in regulating cell survival, proliferation and migration, and its overexpression and activation has been correlated with cancer progression. Cancer therapies targeting EGFR have been applied in the clinic with some success. We show, by confocal microscopy analysis, that illumination of adenocarcinomic human alveolar basal epithelial cells (Human A549-EGFR biosensor cell line) with 280 nm at irradiance levels up to 20 times weaker than the Ultraviolet B (UVB) solar output for short periods of time (15-45 minutes) prevents epidermal growth factor-mediated activation of EGFR located on the cell membrane, preventing or reducing cellular disaggregation, formation of filopodia and cell migration. This effect of Ultraviolet (UV) light illumination was confirmed further in a functional scratch assay, and shown to be more effective than that of a specific EGFR-signaling inhibitor. This new photonic approach may be applicable to the treatment of various types of cancer, alone or in combination with other therapies.
AB - Epidermal growth factor receptor (EGFR) plays a key role in regulating cell survival, proliferation and migration, and its overexpression and activation has been correlated with cancer progression. Cancer therapies targeting EGFR have been applied in the clinic with some success. We show, by confocal microscopy analysis, that illumination of adenocarcinomic human alveolar basal epithelial cells (Human A549-EGFR biosensor cell line) with 280 nm at irradiance levels up to 20 times weaker than the Ultraviolet B (UVB) solar output for short periods of time (15-45 minutes) prevents epidermal growth factor-mediated activation of EGFR located on the cell membrane, preventing or reducing cellular disaggregation, formation of filopodia and cell migration. This effect of Ultraviolet (UV) light illumination was confirmed further in a functional scratch assay, and shown to be more effective than that of a specific EGFR-signaling inhibitor. This new photonic approach may be applicable to the treatment of various types of cancer, alone or in combination with other therapies.
KW - EGF
KW - EGFR
KW - arrest cancer cell migration
KW - metastasis
KW - photonic cancer therapy
KW - protein photochemistry
UR - http://www.scopus.com/inward/record.url?scp=85052843847&partnerID=8YFLogxK
U2 - 10.1002/jbio.201700323
DO - 10.1002/jbio.201700323
M3 - Journal article
C2 - 29537137
SN - 1864-063X
VL - 11
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 9
M1 - e201700323
ER -