TY - JOUR
T1 - Protein-SIP in environmental studies
AU - Jehmlich, Nico
AU - Vogt, Carsten
AU - Lünsmann, Vanessa
AU - Richnow, Hans-Hermann
AU - von Bergen, Martin
PY - 2016
Y1 - 2016
N2 - Metaproteomics coupled to stable isotope probing (SIP) was established to detect metabolically active key players in microbial communities. Here, we discuss the current state of protein-based stable isotope probing (protein-SIP) and the perspectives of using different stable isotope atoms (i.e. 13C, 15N, 18O, 34/36S), multiple isotope labelling, the utilisation of substrates of major abundance and micro-pollutants [pesticides, herbicides and pharmaceuticals present in the environment at very low concentrations (ng μg/L)], and applications in complex model systems and in situ studies in the environment.
AB - Metaproteomics coupled to stable isotope probing (SIP) was established to detect metabolically active key players in microbial communities. Here, we discuss the current state of protein-based stable isotope probing (protein-SIP) and the perspectives of using different stable isotope atoms (i.e. 13C, 15N, 18O, 34/36S), multiple isotope labelling, the utilisation of substrates of major abundance and micro-pollutants [pesticides, herbicides and pharmaceuticals present in the environment at very low concentrations (ng μg/L)], and applications in complex model systems and in situ studies in the environment.
UR - http://www.scopus.com/inward/record.url?scp=84963964188&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2016.04.010
DO - 10.1016/j.copbio.2016.04.010
M3 - Review article
AN - SCOPUS:84963964188
SN - 0958-1669
VL - 41
SP - 26
EP - 33
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
ER -