It is believed that mechanical stimuli of the human gut activate afferents responding either to noxious or normal, physiological stimuli. They may be able to sensitize without relation to the contractile state of the smooth muscle. The current study aimed to verify the above characteristics using a statistical model based on correlation analysis. A method to evaluate the multi-layer stress-strain relation, multi-directional deformation, and the distension-induced sensation in the human esophagus may be valuable for understanding esophageal biomechanics and mechano-sensation.