TY - CHAP
T1 - Allelic Discrimination by TaqMan-PCR for Genotyping of Human Neutrophil Antigens
AU - Steffensen, Rudi
AU - Baech, John
AU - Nielsen, Kaspar R
PY - 2015
Y1 - 2015
N2 - Neutrophil antigens are implicated in a variety of clinical conditions, including neonatal immune neutropenia, transfusion-related acute lung injury, refractoriness to granulocyte transfusions, febrile transfusion reactions, and autoimmune neutropenia. In this report, we describe simultaneous genotyping of human neutrophil antigens (HNA)-1, -3, -4, and -5 using PCR with allele-specific TaqMan probes and end-point fluorescence detection, which is a robust, rapid, and reproducible method, allowing for high-throughput genotyping.
AB - Neutrophil antigens are implicated in a variety of clinical conditions, including neonatal immune neutropenia, transfusion-related acute lung injury, refractoriness to granulocyte transfusions, febrile transfusion reactions, and autoimmune neutropenia. In this report, we describe simultaneous genotyping of human neutrophil antigens (HNA)-1, -3, -4, and -5 using PCR with allele-specific TaqMan probes and end-point fluorescence detection, which is a robust, rapid, and reproducible method, allowing for high-throughput genotyping.
U2 - 10.1007/978-1-4939-2690-9_17
DO - 10.1007/978-1-4939-2690-9_17
M3 - Book chapter
C2 - 26024637
SN - 978-1-4939-2689-3
T3 - Methods in Molecular Biology
SP - 205
EP - 212
BT - Molecular Typing of Blood Cell Antigens
A2 - Bugert, Peter
PB - Springer
ER -