Black perithecial pigmentation in Fusarium species is due to the accumulation of 5-deoxybostrycoidin-based melanin

Rasmus J N Frandsen, Silas A Rasmussen, Peter B Knudsen, Silvio Uhlig, Dirk Petersen, Erik Lysøe, Charlotte H Gotfredsen, Henriette Giese, Thomas O Larsen

Research output: Contribution to journalJournal articleResearchpeer-review

39 Citations (Scopus)

Abstract

Biosynthesis of the black perithecial pigment in the filamentous fungus Fusarium graminearum is dependent on the polyketide synthase PGL1 (oPKS3). A seven-membered PGL1 gene cluster was identified by over-expression of the cluster specific transcription factor pglR. Targeted gene replacement showed that PGL1, pglJ, pglM and pglV were essential for the production of the perithecial pigment. Over-expression of PGL1 resulted in the production of 6-O-demethyl-5-deoxybostrycoidin (1), 5-deoxybostrycoidin (2), and three novel compounds 5-deoxybostrycoidin anthrone (3), 6-O-demethyl-5-deoxybostrycoidin anthrone (4) and purpurfusarin (5). The novel dimeric bostrycoidin purpurfusarin (5) was found to inhibit the growth of Candida albicans with an IC50 of 8.0 +/- 1.9 μM. The results show that Fusarium species with black perithecia have a previously undescribed form of 5-deoxybostrycoidin based melanin in their fruiting bodies.

Original languageEnglish
JournalScientific Reports
Volume6
Pages (from-to)26206
Number of pages6
ISSN2045-2322
DOIs
Publication statusPublished - 19 May 2016

Keywords

  • Journal Article

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