TY - JOUR
T1 - Butyric acid fermentation from pretreated and hydrolysed wheat straw by an adapted Clostridium tyrobutyricum strain
AU - Baroi, G. N.
AU - Baumann, I.
AU - Westermann, P.
AU - Gavala, H. N.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Butyric acid is a valuable building-block for the production of chemicals and materials and nowadays it is produced exclusively from petroleum. The aim of this study was to develop a suitable and robust strain of Clostridium tyrobutyricum that produces butyric acid at a high yield and selectivity from lignocellulosic biomasses. Pretreated (by wet explosion) and enzymatically hydrolysed wheat straw (PHWS), rich in C6 and C5 sugars (71.6 and 55.4gl-1 of glucose and xylose respectively), was used as substrate. After one year of serial selections, an adapted strain of C.tyrobutyricum was developed. The adapted strain was able to grow in 80% (vv-1) PHWS without addition of yeast extract compared with an initial tolerance to less than 10% PHWS and was able to ferment both glucose and xylose. It is noticeable that the adapted C.tyrobutyricum strain was characterized by a high yield and selectivity to butyric acid. Specifically, the butyric acid yield at 60-80% PHWS lie between 0.37 and 0.46gg-1 of sugar, while the selectivity for butyric acid was as high as 0.9-1.0gg-1 of acid. Moreover, the strain exhibited a robust response in regards to growth and product profile at pH 6 and 7.
AB - Butyric acid is a valuable building-block for the production of chemicals and materials and nowadays it is produced exclusively from petroleum. The aim of this study was to develop a suitable and robust strain of Clostridium tyrobutyricum that produces butyric acid at a high yield and selectivity from lignocellulosic biomasses. Pretreated (by wet explosion) and enzymatically hydrolysed wheat straw (PHWS), rich in C6 and C5 sugars (71.6 and 55.4gl-1 of glucose and xylose respectively), was used as substrate. After one year of serial selections, an adapted strain of C.tyrobutyricum was developed. The adapted strain was able to grow in 80% (vv-1) PHWS without addition of yeast extract compared with an initial tolerance to less than 10% PHWS and was able to ferment both glucose and xylose. It is noticeable that the adapted C.tyrobutyricum strain was characterized by a high yield and selectivity to butyric acid. Specifically, the butyric acid yield at 60-80% PHWS lie between 0.37 and 0.46gg-1 of sugar, while the selectivity for butyric acid was as high as 0.9-1.0gg-1 of acid. Moreover, the strain exhibited a robust response in regards to growth and product profile at pH 6 and 7.
UR - http://www.scopus.com/inward/record.url?scp=84938949962&partnerID=8YFLogxK
U2 - 10.1111/1751-7915.12304
DO - 10.1111/1751-7915.12304
M3 - Journal article
AN - SCOPUS:84938949962
SN - 1751-7907
VL - 8
SP - 874
EP - 882
JO - Microbial Biotechnology
JF - Microbial Biotechnology
IS - 5
ER -