Discrimination of PCR products by colour and size improves the accuracy of sex-typing in avian species

Nadia Mucci; Chiara Mengoni; Ettore Randi

doi:10.1007/s12686-016-0623-y

Discrimination of PCR products by colour and size improves the accuracy of sex-typing in avian species

Nadia Mucci^*, Chiara Mengoni, Ettore Randi

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

5 Citations (Scopus)

Abstract

Gender determination of endangered birds with no sexual dimorphism is needed in captive-breeding and conservation projects. Several molecular approaches have been developed, focusing on different species. In this study, we successfully tested a protocol for sex identification, based on the amplification of fluorescently labeled products in 63 species listed in the Convention of Washington (CITES) Appendix I, and belonging to Psittaciformes, Accipitriformes, Falconiformes, Strigiformes, Pelecaniformes, Ciconiiformes, Gruiformes, Passeriformes, and Sphenisciformes. We designed a multiplexed PCR that produces a single amplicon in males and two distinct amplicons, respectively coloured in blue and green, in females. This procedure generates digitally coloured electropherograms that yield a reliable and quick sexing.

Original language	English
Journal	Conservation Genetics Resources
Volume	9
Issue number	1
Pages (from-to)	73-77
Number of pages	5
ISSN	1877-7252
DOIs	https://doi.org/10.1007/s12686-016-0623-y
Publication status	Published - 1 Mar 2017

Keywords

Accipitriformes
Ciconiiformes
CITES
Falconiformes
Gruiformes
Molecular sexing
Pelecaniformes
Psittaciformes
Sphenisciformes
Strigiformes

Access to Document

10.1007/s12686-016-0623-y

AUB Link

Search for the material in Aalborg University Library's search engine

Cite this

@article{7159ee2bfa8041a6ae4a3f5f448016da,

title = "Discrimination of PCR products by colour and size improves the accuracy of sex-typing in avian species",

abstract = "Gender determination of endangered birds with no sexual dimorphism is needed in captive-breeding and conservation projects. Several molecular approaches have been developed, focusing on different species. In this study, we successfully tested a protocol for sex identification, based on the amplification of fluorescently labeled products in 63 species listed in the Convention of Washington (CITES) Appendix I, and belonging to Psittaciformes, Accipitriformes, Falconiformes, Strigiformes, Pelecaniformes, Ciconiiformes, Gruiformes, Passeriformes, and Sphenisciformes. We designed a multiplexed PCR that produces a single amplicon in males and two distinct amplicons, respectively coloured in blue and green, in females. This procedure generates digitally coloured electropherograms that yield a reliable and quick sexing.",

keywords = "Accipitriformes, Ciconiiformes, CITES, Falconiformes, Gruiformes, Molecular sexing, Pelecaniformes, Psittaciformes, Sphenisciformes, Strigiformes",

author = "Nadia Mucci and Chiara Mengoni and Ettore Randi",

year = "2017",

month = mar,

day = "1",

doi = "10.1007/s12686-016-0623-y",

language = "English",

volume = "9",

pages = "73--77",

journal = "Conservation Genetics Resources",

issn = "1877-7252",

publisher = "Springer",

number = "1",

}

TY - JOUR

T1 - Discrimination of PCR products by colour and size improves the accuracy of sex-typing in avian species

AU - Mucci, Nadia

AU - Mengoni, Chiara

AU - Randi, Ettore

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Gender determination of endangered birds with no sexual dimorphism is needed in captive-breeding and conservation projects. Several molecular approaches have been developed, focusing on different species. In this study, we successfully tested a protocol for sex identification, based on the amplification of fluorescently labeled products in 63 species listed in the Convention of Washington (CITES) Appendix I, and belonging to Psittaciformes, Accipitriformes, Falconiformes, Strigiformes, Pelecaniformes, Ciconiiformes, Gruiformes, Passeriformes, and Sphenisciformes. We designed a multiplexed PCR that produces a single amplicon in males and two distinct amplicons, respectively coloured in blue and green, in females. This procedure generates digitally coloured electropherograms that yield a reliable and quick sexing.

AB - Gender determination of endangered birds with no sexual dimorphism is needed in captive-breeding and conservation projects. Several molecular approaches have been developed, focusing on different species. In this study, we successfully tested a protocol for sex identification, based on the amplification of fluorescently labeled products in 63 species listed in the Convention of Washington (CITES) Appendix I, and belonging to Psittaciformes, Accipitriformes, Falconiformes, Strigiformes, Pelecaniformes, Ciconiiformes, Gruiformes, Passeriformes, and Sphenisciformes. We designed a multiplexed PCR that produces a single amplicon in males and two distinct amplicons, respectively coloured in blue and green, in females. This procedure generates digitally coloured electropherograms that yield a reliable and quick sexing.

KW - Accipitriformes

KW - Ciconiiformes

KW - CITES

KW - Falconiformes

KW - Gruiformes

KW - Molecular sexing

KW - Pelecaniformes

KW - Psittaciformes

KW - Sphenisciformes

KW - Strigiformes

UR - http://www.scopus.com/inward/record.url?scp=85016005133&partnerID=8YFLogxK

U2 - 10.1007/s12686-016-0623-y

DO - 10.1007/s12686-016-0623-y

M3 - Journal article

AN - SCOPUS:85016005133

SN - 1877-7252

VL - 9

SP - 73

EP - 77

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - 1

ER -

Discrimination of PCR products by colour and size improves the accuracy of sex-typing in avian species

Abstract

Keywords

Access to Document

AUB Link

Other files and links

Fingerprint

Cite this