Abstract
SEPTIN9 (SEPT9) is a filament-forming protein involved in numerous cellular processes. We have used a conditional knock out allele of Sept9 to specifically delete Sept9 in T-cells. As shown by fluorescence-activated cell sorting, loss of Sept9 at an early thymocyte stage in the thymus results in increased numbers of double-negative cells indicating that SEPT9 is involved in the transition from the double-negative stage during T-cell development. Accordingly, the relative numbers of mature T-cells in the periphery are decreased in mice with a T-cell-specific deletion of Sept9. Proliferation of Sept9-deleted CD8(+) T-cells from the spleen is decreased upon stimulation in culture. The altered T-cell homeostasis caused by the loss of Sept9 results in an increase of CD8(+) central memory T-cells.
Original language | English |
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Journal | Cell and Tissue Research |
Volume | 352 |
Issue number | 3 |
Pages (from-to) | 695-705 |
Number of pages | 11 |
ISSN | 0302-766X |
DOIs | |
Publication status | Published - Jun 2013 |
Keywords
- Animals
- CD8-Positive T-Lymphocytes
- Cell Count
- Cell Differentiation
- Cell Proliferation
- Down-Regulation
- Homeostasis
- Immunologic Memory
- Integrases
- Lymphocyte Depletion
- Lymphoid Tissue
- Mice
- Mice, Knockout
- Real-Time Polymerase Chain Reaction
- Septins
- Up-Regulation