Flow cytometry-assisted cloning of specific sequence motifs from complex 16S rRNA gene libraries

J. L. Nielsen; A. Schramm; G. van den Engh; D. A. Stahl

doi:10.1128/AEM.70.12.7550-7554.2004

Flow cytometry-assisted cloning of specific sequence motifs from complex 16S rRNA gene libraries

J. L. Nielsen, A. Schramm, G. van den Engh, D. A. Stahl

Department of Chemistry and Bioscience

Research output: Contribution to journal › Journal article › Research › peer-review

9 Citations (Scopus)

Abstract

A How cytometry method was developed for rapid screening and recovery of cloned DNA containing common sequence motifs. This approach, termed fluorescence-activated cell sorting-assisted cloning, was used to recover sequences affiliated with a unique lineage within the Bacteroidetes not abundant in a clone library of environmental 16S rRNA genes.

Original language	English
Journal	Applied and Environmental Microbiology
Volume	70
Issue number	12
Pages (from-to)	7550-7554
Number of pages	5
ISSN	0099-2240
DOIs	https://doi.org/10.1128/AEM.70.12.7550-7554.2004
Publication status	Published - Dec 2004

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Cite this

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title = "Flow cytometry-assisted cloning of specific sequence motifs from complex 16S rRNA gene libraries",

abstract = "A How cytometry method was developed for rapid screening and recovery of cloned DNA containing common sequence motifs. This approach, termed fluorescence-activated cell sorting-assisted cloning, was used to recover sequences affiliated with a unique lineage within the Bacteroidetes not abundant in a clone library of environmental 16S rRNA genes.",

author = "Nielsen, {J. L.} and A. Schramm and Engh, {G. van den} and Stahl, {D. A.}",

year = "2004",

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AU - Schramm, A.

AU - Engh, G. van den

AU - Stahl, D. A.

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N2 - A How cytometry method was developed for rapid screening and recovery of cloned DNA containing common sequence motifs. This approach, termed fluorescence-activated cell sorting-assisted cloning, was used to recover sequences affiliated with a unique lineage within the Bacteroidetes not abundant in a clone library of environmental 16S rRNA genes.

AB - A How cytometry method was developed for rapid screening and recovery of cloned DNA containing common sequence motifs. This approach, termed fluorescence-activated cell sorting-assisted cloning, was used to recover sequences affiliated with a unique lineage within the Bacteroidetes not abundant in a clone library of environmental 16S rRNA genes.

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DO - 10.1128/AEM.70.12.7550-7554.2004

M3 - Journal article

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JF - Applied and Environmental Microbiology

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