Activities per year
Abstract
Interleukin 1α (IL-1α) is a proinflammatory cytokine that belongs to the IL-1 family. It is produced mainly by macrophages at sites of infection and regarded as an essential regulator of acute inflammation. IL-1α is synthesized as a 33 kDa precursor peptide that is cleaved by a calpain-like protease to a nuclear -associated 16 kDa propiece and a secreted 17 kDa mature IL-1α peptide. However, the full understanding of its dual function is missing. Recently, SNPs in the gene for IL-1α was also associated with the risk of developing ankylosing spondylitis (AS), a subgroup of the spondyloarthropathies. These findings lead us to produce antibodies towards the N- and C-terminal region of IL-1α to investigate IL-1α kinetics in human macrophages. This would eventually be used to asses any correlation between a defect in the production of the cytokine and a disease related SNP found in the IL-1α gene in patients suffering from AS. In the present study we generated human macrophages (Mf) from blood monocytes, stimulated the cells with lipopolysaccharide (LPS) and analysed the production and localization of IL-1α by use of monoclonal antibodies (MAbs) generated against recombinant precursor IL-1α. We obtained a MAb specific for the N-terminal propiece and for the C-terminal mature form of IL-1α, respectively. Assays, including DNA sequencing, immunofluorescence microscopy, qPCR and FACS are now available for the analysis of IL-1α kinetics in blood samples from AS patients.
Original language | English |
---|---|
Journal | Orthopedic & Muscular System : Current Research |
Volume | 3 |
Issue number | 2 |
Pages (from-to) | 89 |
ISSN | 2161-0533 |
DOIs | |
Publication status | Published - 2014 |
Event | International Conference on Orthopedics & Rheumatology - San Francisco, CA, United States Duration: 28 Jul 2014 → 30 Jul 2014 Conference number: 3 |
Conference
Conference | International Conference on Orthopedics & Rheumatology |
---|---|
Number | 3 |
Country/Territory | United States |
City | San Francisco, CA |
Period | 28/07/2014 → 30/07/2014 |
Keywords
- Spondyloarthritis
- Interleukin-1 alpha
- Kinetics
Fingerprint
Dive into the research topics of 'Can a functional assay on cytokine kinetics be used for the identification of a disease-related role for Single Nucleotide Polymorphisms (SNPs) in Ankolysing spondylitis?'. Together they form a unique fingerprint.Activities
- 1 Membership of research networks or expert groups
-
La Jolla Institute for Allergy and Immunology (External organisation)
Thomas Gelsing Carlsen (Member)
1 Apr 2014 → 1 Jul 2014Activity: Memberships › Membership of research networks or expert groups
File