Microscale structure and function of anaerobic-aerobic granules containing glycogen accumulating organisms
Publication: Research - peer-review › Journal article
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The spatial arrangement and metabolic activity of 'Candidatus
Competibacter phosphatis' was investigated in granular sludge from an
anaerobic-aerobic sequencing batch reactor enriched for
glycogen-accumulating organisms. In this process, the electron donor
(acetate) and the electron acceptor (oxygen) were supplied sequentially
in each phase. The organism, identified by fluorescence in situ
hybridisation, was present throughout the granules; however, metabolic
activity was limited to a 100-mum-thick layer immediately below the
surface of the granules. To investigate the cause of this, oxygen
microsensors and a novel microscale biosensor for volatile fatty acids
were used in conjunction with chemical staining for intracellular
storage polymers. It was found that the limited distribution of
activity was caused by mass transport limitation of oxygen into the
granules during the aerobic phase.
| Original language | English |
|---|---|
| Journal | FEMS Microbiology Ecology |
| Publication date | 2003 |
| Volume | 45 |
| Journal number | 3 |
| Pages | 253-261 |
| ISSN | 0168-6496 |
| DOIs | |
| State | Published |
ID: 18237153