TY - JOUR
T1 - Optimized production of Aspergillus aculeatus URM4953 polygalacturonases for pectin hydrolysis in hog plum (Spondias mombin L.) juice
AU - Silva, Jônatas de Carvalho
AU - de França, Pedro Renann Lopes
AU - de Melo, Allan Henrique Felix
AU - Neves-Petersen, Maria Teresa
AU - Converti, Attilio
AU - Souza Porto, Tatiana
PY - 2019/4
Y1 - 2019/4
N2 - Production of total polygalacturonase (PG) and endo-polygalacturonases (endo-PG) from Aspergillus aculeatus URM4953 was optimized in submerged fermentation using passion fruit peel as substrate for pectin hydrolysis in hog plum juice. The highest activities of PG (2.92 ± 0.12 U/mL) and endo-PG (6.51 ± 0.04 U/mL) were obtained at 30 °C, pH 4.56 and 130 rpm, using 3.0% substrate and 0.1% yeast extract after 96 h in 250-mL Erlenmeyer flasks. Under these optimized conditions, maximum specific growth rate of the microorganism was 0.06 h−1, saturation constant 9.9 mg/mL, yield of biomass on consumed substrate 1.44 g/g, yields of PG and endo-PG on consumed substrate 0.33 and 0.81 U/mg, and yields of PG and endo-PG on biomass 0.45 and 0.95 U/mg, respectively. Endo-PG, which is responsible for reduction of fruit juice viscosity, displayed an optimum temperature of 60 °C and two optimal pH values (5.0 and 7.0). A novel zymography method showed PG activity in correspondence to a protein band of 31.7 kDa. The enzyme mixture was used for pectin hydrolysis in hog plum juice, which occurred optimally at 40 °C, achieving a juice yield of 35.3% and reducing fruit viscosity by 96.8% within 88 min. A. aculeatus polygalacturonases have been demonstrated to have great industrial potential for pectin hydrolysis in fruit juices.
AB - Production of total polygalacturonase (PG) and endo-polygalacturonases (endo-PG) from Aspergillus aculeatus URM4953 was optimized in submerged fermentation using passion fruit peel as substrate for pectin hydrolysis in hog plum juice. The highest activities of PG (2.92 ± 0.12 U/mL) and endo-PG (6.51 ± 0.04 U/mL) were obtained at 30 °C, pH 4.56 and 130 rpm, using 3.0% substrate and 0.1% yeast extract after 96 h in 250-mL Erlenmeyer flasks. Under these optimized conditions, maximum specific growth rate of the microorganism was 0.06 h−1, saturation constant 9.9 mg/mL, yield of biomass on consumed substrate 1.44 g/g, yields of PG and endo-PG on consumed substrate 0.33 and 0.81 U/mg, and yields of PG and endo-PG on biomass 0.45 and 0.95 U/mg, respectively. Endo-PG, which is responsible for reduction of fruit juice viscosity, displayed an optimum temperature of 60 °C and two optimal pH values (5.0 and 7.0). A novel zymography method showed PG activity in correspondence to a protein band of 31.7 kDa. The enzyme mixture was used for pectin hydrolysis in hog plum juice, which occurred optimally at 40 °C, achieving a juice yield of 35.3% and reducing fruit viscosity by 96.8% within 88 min. A. aculeatus polygalacturonases have been demonstrated to have great industrial potential for pectin hydrolysis in fruit juices.
KW - Aspergillus aculeatus
KW - Hog plum juice (Spondias mombin L.)
KW - Optimization
KW - Pectin hydrolysis
KW - Polygalacturonases
UR - http://www.scopus.com/inward/record.url?scp=85059702124&partnerID=8YFLogxK
U2 - 10.1016/j.procbio.2018.12.014
DO - 10.1016/j.procbio.2018.12.014
M3 - Journal article
AN - SCOPUS:85059702124
SN - 1359-5113
VL - 79
SP - 18
EP - 27
JO - Process Biochemistry
JF - Process Biochemistry
ER -