Activities per year
Abstract
Few‐atom silver nanoclusters (AgNCs) can exhibit strong fluorescence; however, they require ligands to prevent aggregation into larger nanoparticles. Fluorescent AgNCs in biopolymer scaffolds have so far mainly been synthesized in solution, and peptides have only found limited use compared to DNA. Herein, we demonstrate how solid‐phase methods can increase throughput dramatically in peptide ligand screening and in initial evaluation of fluorescence intensity and chemical stability of peptide‐stabilized AgNCs (P‐AgNCs). 9‐Fluorenylmethyloxycarbonyl (Fmoc) solid‐phase peptide synthesis on a hydroxymethyl‐benzoic acid (HMBA) polyethylene glycol polyacrylamide copolymer (PEGA) resin enabled on‐resin screening and evaluation of a peptide library, leading to identification of novel peptide‐stabilized, fluorescent AgNCs. Using systematic amino acid substitutions, we synthesized and screened a 144‐member library. This allowed us to evaluate the effect of length, charge, and Cys content in peptides used as ligands for AgNC stabilization. The results of this study will enable future spectroscopic studies of these peptide‐stabilized AgNCs for bioimaging and other applications.
Original language | English |
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Article number | 8 |
Journal | Chemistry: A European Journal |
Volume | 22 |
Issue number | 51 |
Pages (from-to) | 18492 |
Number of pages | 18,500 |
ISSN | 0947-6539 |
DOIs | |
Publication status | Published - 3 Nov 2016 |
Externally published | Yes |
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Dive into the research topics of 'Peptide‐Stabilized, Fluorescent Silver Nanoclusters: Solid‐Phase Synthesis and Screening'. Together they form a unique fingerprint.Activities
- 1 Conference presentations
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23rd American Peptide Symposium
Simon Gregersen (Speaker)
22 Jun 2013 → 27 Jun 2013Activity: Talks and presentations › Conference presentations
Prizes
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Flourescent peptide-stabilized silver-nanoclusters, a solid-phase approach for high-throughput ligand discovery
Gregersen, S., 4 Feb 2014, 1 ed. Copenhagen: Copenhagen University. 172 p.Research output: PhD thesis
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Novel Peptide Ligands for Stabilization of Fluorescent, Silver Nanoclusters: On-resin Screening of a Peptide Library
Gregersen, S., Jensen, K. J. & Vosch, T. A. J., 13 May 2013.Research output: Contribution to conference without publisher/journal › Poster › Research › peer-review
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Peptides as Ligands for Fluorescent, Silver Nanoclusters: Development of a Novel Platform for On-resin Screening
Gregersen, S., Jensen, K. J. & Vosch, T. A. J., 13 May 2013, In: Journal of Peptide Science. 100, p. 294 1 p., YI-P 216.Research output: Contribution to journal › Conference abstract in journal › Research › peer-review
Open Access