Post Mortem Leukocyte Scintigraphy in Juvenile Pigs with Experimentally Induced Osteomyelitis

P. Afzelius, O. L. Nielsen, S. B. Jensen, A. K. O. Alstrup

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1 Citation (Scopus)

Abstract

We have previously demonstrated that In-111-labeled autologous leukocyte scintigraphy is able to detect osteomyelitis in living juvenile pigs. In animal research studies, it may well be an advantage if the animals could be scanned after euthanasia. Applying traditional scanning of living animals to euthanized animals will render anaesthesia unnecessary and be ideal for obtaining good and reliable scans for the correct interpretation of imaging afterwards, since the animals do not move. The autologous leukocytes of the pigs were collected, marked with In-111, and reinjected into the pigs and allowed for homing to the site of infections as usual while the pigs were alive. In this study, we demonstrate that it is possible to perform SPECT/CT with In-111-labelled autologous leukocytes almost 24 hrs after euthanasia with the same detectability of osteomyelitic lesions as in living pigs (78% versus 79%). The pigs in this study had exactly the same experimental conditions as the living pigs and were examined in parallel with the living pigs except for euthanasia prior to the leukocyte scan and that no PET/CT scans were performed.
Original languageEnglish
Article number3603929
JournalContrast Media & Molecular Imaging (Online)
Volume2017
Number of pages6
ISSN1555-4317
DOIs
Publication statusPublished - 2017

Bibliographical note

ISI Document Delivery No.: FI0MK Times Cited: 0 Cited Reference Count: 9 Afzelius, Pia Nielsen, Ole Lerberg Jensen, Svend Borup Alstrup, Aage Kristian Olsen Danish Council for Independent Research, Technology and Production Sciences [0602-01911B (11107077)] This work was supported by Grant no. 0602-01911B (11107077) from the Danish Council for Independent Research, Technology and Production Sciences. The authors are grateful for the technical support provided by Dennis Brok, Rikke Skall, Janne Frederiksen, and Lotte S. Meyer. 0 1 Wiley-hindawi London 1555-4317

Keywords

  • hematogenous osteomyelitis porcine model c-11-methionine f-18-fdg ga-68-citrate c-11-pk11195 Radiology, Nuclear Medicine & Medical Imaging

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