TY - JOUR
T1 - Verticillium dahliae disease resistance and the regulatory pathway for maturity and tuberization in potato
AU - Tai, Helen H.
AU - De Koyer, David
AU - Sønderkær, Mads
AU - Hedegaard, Sanne
AU - Lague, Martin
AU - Goyer, Claudia
AU - Nolan, Lana
AU - Davidson, Charlotte
AU - Gardner, Kyle
AU - Neilson, Jonathan
AU - Paudel, Jamuna Risal
AU - Murphy, Agnes
AU - Bizimungu, Benoit
AU - Wang, Hui Ying
AU - Xiong, Xingyao
AU - Halterman, Dennis
AU - Nielsen, Kåre Lehmann
PY - 2018
Y1 - 2018
N2 - Verticillium dahliae Kleb. is a pathogenic fungus causing wilting, chlorosis, and early dying in potato (Solanum tuberosum L.). Genetic mapping of resistance to V. dahliae was done using a diploid population of potato. The major quantitative trait locus (QTL) for Verticillium resistance was found on chromosome 5. The StCDF1 gene, controlling earliness of maturity and tuberization, was mapped within the interval. Another QTL on chromosome 9 co-localized with the Ve2 Verticillium wilt resistance gene marker. Epistasis analysis indicated that the loci on chromosomes 5 and 9 had a highly significant interaction, and that StCDF1 functioned downstream of Ve2. The StCDF1 alleles were sequenced and found to encode StCDF1.1 and StCDF1.3. Interaction between the Ve2 resistance allele and the StCDF1.3 was demonstrated, but not for StCDF1.1. Genome-wide expression QTL (eQTL) analysis was performed and genes with eQTL at the StCDF1 and Ve2 loci were both found to have similar functions involving the chloroplast, including photosynthesis, which declines in both maturity and Verticillium wilt. Among the gene ontology (GO) terms that were specific to genes with eQTL at the Ve2, but not the StCDF1 locus, were those associated with fungal defense. These results suggest that Ve2 controls fungal defense and reduces early dying in Verticillium wilt through affecting genetic pathway controlling tuberization timing.
AB - Verticillium dahliae Kleb. is a pathogenic fungus causing wilting, chlorosis, and early dying in potato (Solanum tuberosum L.). Genetic mapping of resistance to V. dahliae was done using a diploid population of potato. The major quantitative trait locus (QTL) for Verticillium resistance was found on chromosome 5. The StCDF1 gene, controlling earliness of maturity and tuberization, was mapped within the interval. Another QTL on chromosome 9 co-localized with the Ve2 Verticillium wilt resistance gene marker. Epistasis analysis indicated that the loci on chromosomes 5 and 9 had a highly significant interaction, and that StCDF1 functioned downstream of Ve2. The StCDF1 alleles were sequenced and found to encode StCDF1.1 and StCDF1.3. Interaction between the Ve2 resistance allele and the StCDF1.3 was demonstrated, but not for StCDF1.1. Genome-wide expression QTL (eQTL) analysis was performed and genes with eQTL at the StCDF1 and Ve2 loci were both found to have similar functions involving the chloroplast, including photosynthesis, which declines in both maturity and Verticillium wilt. Among the gene ontology (GO) terms that were specific to genes with eQTL at the Ve2, but not the StCDF1 locus, were those associated with fungal defense. These results suggest that Ve2 controls fungal defense and reduces early dying in Verticillium wilt through affecting genetic pathway controlling tuberization timing.
UR - http://www.scopus.com/inward/record.url?scp=85043777259&partnerID=8YFLogxK
U2 - 10.3835/plantgenome2017.05.0040
DO - 10.3835/plantgenome2017.05.0040
M3 - Journal article
SN - 1940-3372
VL - 11
SP - 1
JO - The Plant Genome
JF - The Plant Genome
IS - 1
M1 - 170040
ER -