Beskrivelse
Introduction and Objectives (300 words combined max) Inflammatory bowel diseases, including Ulcerative colitis affects 2.5–3 million people in Europe. The life-long conditions significantly reduce the quality of life of the patients, and compose a significant economic burden for society. The etiology of these diseases remains incompletely explained. We, therefore, performed the most thorough proteome-based study of ulcerative colitis colon tissue, based on number of analyzed proteins, and have for the first time observed neutrophil extracellular traps in ulcerative colitis, detected by proteomics and verified by microscopy. Methods We performed a comparative proteome analysis of non-inflamed mucosal colon biopsies from 10 patients with ulcerative colitis and 10 controls. The proteome of the biopsies were characterized by high throughput label-free quantitative proteomics, and the biopsy histology was analyzed by microscopy. Results and Discussion We quantified 5,711 different proteins in the colon biopsies. Forty-six proteins had a statistically significant difference in abundance between the ulcerative colitis colon tissue and controls. Eleven of the proteins with increased abundances in the ulcerative colitis biopsies were associated with neutrophils and neutrophil extracellular traps. The abundance of calprotectin and lactotransferrin in the tissue correlated with the degree of tissue inflammation as determined by histology. The findings were validated by microscopy where an increased abundance of neutrophils within the mucosal tissue was found. Additionally, we confirmed the presence of neutrophil extracellular traps by extracellular DNA in the ulcerative colitis colon tissue. Conclusion We found an increased abundance of several proteins hitherto not associated with innate immunity and neutrophils in non-inflamed mucosal colon biopsies from ulcerative colitis patients. The increased abundance of these antimicrobial compounds points to the stimulation of the innate immune system in the etiology of ulcerative colitis. Our findings demonstrate that even though remission has been achieved on the surface of the ulcerative colitis colon tissue, a chronic condition is still present within the morphologically normal tissue.| Periode | 28 sep. 2015 |
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| Begivenhedstitel | Human Proteome Organization World Congress: HUPO2015 |
| Begivenhedstype | Konference |
| Placering | Vancouver, CanadaVis på kort |