This supplementary dataset contains all supplementary information for manuscript "Genetic Signatures of Drug Response Variability in Drosophila melanogaster". Each file in described in Supplementary File S1. In addition, DGRP genotypes, information on Wolbachia infection status, and chromosomal inversions can be accessed via the website http:dgrp2.gnets.ncsu.edu. The raw and normalized gene expression data of wild type flies are available at Gene Expression Omnibus under accession number GSE121643.