Enzymes from complex microbial systems

Beskrivelse

In complex microbial systems (e.g. wastewater treatment plants), a variety of microorganisms take part in organic matter decomposition. In such systems hydrolytic enzymes catalyze the first and rate limiting reaction. In domestic wastewater treatment plants, up to 60- 70 % of the organic matter in the incoming wastewater is accounted for by proteins and lipids. As a result, our main focus in this study is to understand lipid and protein turnover. We isolate enzymes directly from the environment. After isolation of the organisms that produce the enzymes, we conduct detailed biochemical and biophysical characterization of the protein. The study, apart from providing valuable information about the role of the different organisms and their enzymes in the actual environment, allows us to isolate enzymes with potential for a variety of interesting biotechnological applications. Cloning of the gene(s) coding for these enzymes could enable us to further improve the properties of the enzyme through directed molecular evolution for a particular application condition. (Amare Gessesse, Steffen B. Petersen, Eva I. Petersen; Per H. Nielsen, Section of Environmental Engineering)
StatusAfsluttet
Effektiv start/slut dato31/12/200431/12/2004