In situ characterization of the microbial degraders of xenobiotic compounds from industrial waste and natural environments



Brief description of project: The aim of the project is to introduce a new concept in the study of microorganisms capable of degrading environmental contaminants in enriched reactor cultures. This includes an examination of the organisms which are actively utilising the contaminant by applying Microautoradiography in combination with Fluorescence In Situ Hybridisation (MAR-FISH). The identity established by FISH will be used to sort a clone library by flow cytometry by FACS-Cloning (Nielsen, J.L, Schramm, A., van den Engh, G., and Stahl, D.A., AEM 70:7550). This approach will reveal the identity of true degraders of the contaminant, and design new oligonucleotide probes for usage by FISH. Incubations with radiolabeled isotopes with different electron acceptor conditions and changes in external parameters, such as temperature, specific inhibitors towards specific reactions etc. can be used to investigate the in situ physiology directly in complex microbial systems. This knowledge will establish a fundamental understanding about external parameters influencing the in situ physiology of contaminant-degrading bacteria. This knowledge can be used in bioaugmentation and remediation of industrial wastewater and the concept directly transferred to other types of industries.  
Effektiv start/slut dato01/04/200112/06/2031