TY - JOUR
T1 - Quantum dots-βcyclodextrin-histidine labeled human adipose stem cells-laden chitosan hydrogel for bone tissue engineering
AU - Jahid Zomorrodi, Vahid
AU - Vasheghani-Farahani, Ebrahim
AU - Bagheri, Fatemeh
AU - Zarrabi, Ali
AU - Jensen, Helene Halkjær
AU - Larsen, Kim Lambertsen
PY - 2020
Y1 - 2020
N2 - Mesenchymal stem cells with differentiation ability to diverse cells play a crucial role in tissue engineering. Tracking the fate of these cells during the regeneration of tissue helps to obtain more information about their function. In this study, histidine conjugated β-cyclodextrin as a cell-penetrating carrier with drug loading ability was attached to QDs nanoparticle (QD-βCD-His) for stem cell labeling. Traceability of QD-βCD-His labeled human adipose stem cells (hASCs) was monitored in 2D cell culture and 3D temperature-sensitive chitosan hydrogel scaffold. Dexamethasone (Dex) as an osteoinductive drug was loaded into QD-βCD-His nano-carrier (QD-βCD-His@Dex) to induce bone differentiation of labeled cells. Overall results indicated that QD-βCD-His@Dex is a promising dual-purpose nano-carrier for stem cell labeling with osteoinductive potential in cell therapy as well as tissue engineering scaffolds.
AB - Mesenchymal stem cells with differentiation ability to diverse cells play a crucial role in tissue engineering. Tracking the fate of these cells during the regeneration of tissue helps to obtain more information about their function. In this study, histidine conjugated β-cyclodextrin as a cell-penetrating carrier with drug loading ability was attached to QDs nanoparticle (QD-βCD-His) for stem cell labeling. Traceability of QD-βCD-His labeled human adipose stem cells (hASCs) was monitored in 2D cell culture and 3D temperature-sensitive chitosan hydrogel scaffold. Dexamethasone (Dex) as an osteoinductive drug was loaded into QD-βCD-His nano-carrier (QD-βCD-His@Dex) to induce bone differentiation of labeled cells. Overall results indicated that QD-βCD-His@Dex is a promising dual-purpose nano-carrier for stem cell labeling with osteoinductive potential in cell therapy as well as tissue engineering scaffolds.
KW - Bone differentiation
KW - Cell tracking
KW - Chitosan hydrogel
KW - Nano-carrier
KW - Quantum dots
KW - β-Cyclodextrin
UR - http://www.scopus.com/inward/record.url?scp=85084653171&partnerID=8YFLogxK
U2 - 10.1016/j.nano.2020.102217
DO - 10.1016/j.nano.2020.102217
M3 - Journal article
SN - 1549-9634
VL - 27
JO - Nanomedicine: Nanotechnology, Biology and Medicine
JF - Nanomedicine: Nanotechnology, Biology and Medicine
M1 - 102217
ER -