TY - JOUR
T1 - Fatty acid hydratase for value-added biotransformation
T2 - A review
AU - Zhang, Yan
AU - Eser, Bekir Engin
AU - Kristensen, Peter
AU - Guo, Zheng
PY - 2020/8
Y1 - 2020/8
N2 - The synthesis of hydroxy fatty acids (HFAs) from renewable oil feedstock by addition of water onto C[dbnd]C bonds has attracted great attention in recent years. Given that selective asymmetric hydration of non-activated C[dbnd]C bonds has been proven difficult to achieve with chemical catalysts, enzymatic catalysis by fatty acid hydratases (FAHs) presents an attractive alternative approach to produce value-added HFAs with high regio-, enantio- and stereospecificity, as well as excellent atom economy. Even though FAHs have just been investigated as a potential biocatalyst for a decade, remarkable information about FAHs in different aspects is available; however, a comprehensive review has not been archived. Herein, we summarize the research progresses on biochemical characterization, structural and mechanistic determination, enzyme engineering, as well as biotechnological application of FAHs. The current challenges and opportunities for an efficient utilization of FAHs in organic synthesis and industrial applications are critically discussed.
AB - The synthesis of hydroxy fatty acids (HFAs) from renewable oil feedstock by addition of water onto C[dbnd]C bonds has attracted great attention in recent years. Given that selective asymmetric hydration of non-activated C[dbnd]C bonds has been proven difficult to achieve with chemical catalysts, enzymatic catalysis by fatty acid hydratases (FAHs) presents an attractive alternative approach to produce value-added HFAs with high regio-, enantio- and stereospecificity, as well as excellent atom economy. Even though FAHs have just been investigated as a potential biocatalyst for a decade, remarkable information about FAHs in different aspects is available; however, a comprehensive review has not been archived. Herein, we summarize the research progresses on biochemical characterization, structural and mechanistic determination, enzyme engineering, as well as biotechnological application of FAHs. The current challenges and opportunities for an efficient utilization of FAHs in organic synthesis and industrial applications are critically discussed.
KW - Biotransformation
KW - Catalytic mechanism
KW - Enzyme characterization
KW - Enzyme engineering
KW - Fatty acid hydratases
KW - Hydroxy fatty acids
UR - http://www.scopus.com/inward/record.url?scp=85086593913&partnerID=8YFLogxK
U2 - 10.1016/j.cjche.2020.02.008
DO - 10.1016/j.cjche.2020.02.008
M3 - Review article
AN - SCOPUS:85086593913
SN - 1004-9541
VL - 28
SP - 2051
EP - 2063
JO - Chinese Journal of Chemical Engineering
JF - Chinese Journal of Chemical Engineering
IS - 8
ER -