Abstract
The distribution of GLUT-1 and GLUT-4 in cryosections of rat skeletal muscles was investigated immunocytochemically. Intense labeling of GLUT-1 was found in the perineurial sheaths of intramuscular nerves, whereas only a very faint signal was associated with the sarcolemma, and labeling of extraneural vessels was not detectable. The majority of the GLUT-4 reactivity was located at the periphery of muscle cells in nonuniform patches, and GLUT-4 was absent in vessels and nerves. In sections of femoral nerve GLUT-1 was confined to the perineurial sheath and endoneurial vessels. The contribution of GLUT-1 from intramuscular perineurial sheaths to total GLUT-1 in a muscle was determined from immunoblots of crude membranes isolated from mixtures of homogenates of excised nerves and muscles. The recovery of GLUT-1 increased linearly with the amount of nerve added, and it was calculated that GLUT-1 from intramuscular perineurial sheaths accounted for approximately 60% of the GLUT-1 content in a membrane fraction from soleus muscle or red gastrocnemius. The remaining 40% of GLUT-1 is likely to originate from the sarcolemma.
Originalsprog | Engelsk |
---|---|
Tidsskrift | American Journal of Physiology (Consolidated) |
Vol/bind | 262 |
Udgave nummer | 5 Pt 1 |
Sider (fra-til) | E721-7 |
ISSN | 0002-9513 |
Status | Udgivet - 1992 |
Udgivet eksternt | Ja |
Emneord
- Animals
- Blood Vessels
- Femoral Nerve
- Fluorescent Antibody Technique
- Glucose Transporter Type 1
- Immunohistochemistry
- Male
- Monosaccharide Transport Proteins
- Muscles
- Nervous System
- Rats
- Rats, Inbred Strains