Alteration of Na+ and K+ ion composition of microbial consortium isolated from oil reservoir at high salinities

Svetlana Nikolayevna Rudyk, Erik Gydesen Søgaard

Publikation: Bidrag til bog/antologi/rapport/konference proceedingKonferenceabstrakt i proceedingForskning

Resumé


The microbes being injected into the oil layers for the purpose of Microbial Enhanced Oil Recovery (MEOR) undergo the influence of extreme environment of oil reservoir like high salinity, high temperature and high pressure which can suppress their viability and production of the desired by-products such as gases, acids, surfactants and the others. To plan MEOR projects for implementation in the oil reservoirs in the North Sea fields, it needs to take into consideration that the salinity of the formation waters is high, varies in a wide range and depends on the closeness to the salt domes. For the most of bacteria salinity above 50 g/l would have an inhibiting effect. The limits of the optimal conditions for microbial propagation can be overcome through the adaptation processes the bacteria-MEOR candidates go through during laboratory studying. Alteration of the ionic composition is known as playing role in the adaption to salinity [1-3].
The change in the concentration of Na+ and K+ cations in the microbial solution exposed to higher concentrations of sodium chloride was under investigation. The experiment was conducted with the consortium of microbes isolated from the oil-saturated core sample extracted from the depth of 2 km from Dan field at several NaCl concentrations varying from 40 to 70 g/l. The samples of microbial solutions were taken during 11 days. The concentration of Na+ and K+ cations in the solution were measured using ICP/MS spectrometry, Optima 3000 DV.
The ion concentrations versus time as a function of salinity were plotted for sodium and potassium separately. In general, for both sodium and potassium ions the lowest concentrations were recorded for salinity of 40 g/l, the highest for 70 g/l with 50 g/l and 60 g/l in between. For Na+ concentration that can be explained solely by salt concentration, but for K+ that effect can be just due to the processes that occurred within microbes. Moreover, the K+-concentration significantly increases and Na+-concentration decreases after 8th day of experiment showing that K+ move out of the cell and Na+ move in. Negative correlation between pH change and K+-concentration was also observed.
[1] J. Chr. Skou. (1998). The identification of sodium pump. Nobel Lecture. Bioscience Reports, Vol.18, No.4.
[2] Apte S.K., Reddy B.R., Thomas J.(1987). Relationship between Sodium Influx and Salt Tolerance of Nitrogen-Fixing Cyanobacteria. Appl. Environ. Microbiology. 53(8):1934-1939.
[3] Sheldon Adler and Donald S. Fraley. (1977). Potassium and intracellular pH. Kidney International, Vol.11, pp.433-442.
OriginalsprogEngelsk
Titel8th International Congress on Extremophiles 2010 : Book of Abstracts
Antal sider1
Publikationsdato2010
Sider312
KapitelP211
StatusUdgivet - 2010

Fingerprint

salinity
ion
oil
enhanced oil recovery
sodium
potassium
cation
salt
salt dome
ionic composition
bacterium
formation water
microbiology
sodium chloride
surfactant
spectrometry
cyanobacterium
pump
viability
tolerance

Citer dette

Rudyk, S. N., & Søgaard, E. G. (2010). Alteration of Na+ and K+ ion composition of microbial consortium isolated from oil reservoir at high salinities. I 8th International Congress on Extremophiles 2010: Book of Abstracts (s. 312)
Rudyk, Svetlana Nikolayevna ; Søgaard, Erik Gydesen. / Alteration of Na+ and K+ ion composition of microbial consortium isolated from oil reservoir at high salinities. 8th International Congress on Extremophiles 2010: Book of Abstracts. 2010. s. 312
@inbook{ed638cda2640474a89000cedc0f0dcce,
title = "Alteration of Na+ and K+ ion composition of microbial consortium isolated from oil reservoir at high salinities",
abstract = "The microbes being injected into the oil layers for the purpose of Microbial Enhanced Oil Recovery (MEOR) undergo the influence of extreme environment of oil reservoir like high salinity, high temperature and high pressure which can suppress their viability and production of the desired by-products such as gases, acids, surfactants and the others. To plan MEOR projects for implementation in the oil reservoirs in the North Sea fields, it needs to take into consideration that the salinity of the formation waters is high, varies in a wide range and depends on the closeness to the salt domes. For the most of bacteria salinity above 50 g/l would have an inhibiting effect. The limits of the optimal conditions for microbial propagation can be overcome through the adaptation processes the bacteria-MEOR candidates go through during laboratory studying. Alteration of the ionic composition is known as playing role in the adaption to salinity [1-3]. The change in the concentration of Na+ and K+ cations in the microbial solution exposed to higher concentrations of sodium chloride was under investigation. The experiment was conducted with the consortium of microbes isolated from the oil-saturated core sample extracted from the depth of 2 km from Dan field at several NaCl concentrations varying from 40 to 70 g/l. The samples of microbial solutions were taken during 11 days. The concentration of Na+ and K+ cations in the solution were measured using ICP/MS spectrometry, Optima 3000 DV. The ion concentrations versus time as a function of salinity were plotted for sodium and potassium separately. In general, for both sodium and potassium ions the lowest concentrations were recorded for salinity of 40 g/l, the highest for 70 g/l with 50 g/l and 60 g/l in between. For Na+ concentration that can be explained solely by salt concentration, but for K+ that effect can be just due to the processes that occurred within microbes. Moreover, the K+-concentration significantly increases and Na+-concentration decreases after 8th day of experiment showing that K+ move out of the cell and Na+ move in. Negative correlation between pH change and K+-concentration was also observed.[1] J. Chr. Skou. (1998). The identification of sodium pump. Nobel Lecture. Bioscience Reports, Vol.18, No.4. [2] Apte S.K., Reddy B.R., Thomas J.(1987). Relationship between Sodium Influx and Salt Tolerance of Nitrogen-Fixing Cyanobacteria. Appl. Environ. Microbiology. 53(8):1934-1939.[3] Sheldon Adler and Donald S. Fraley. (1977). Potassium and intracellular pH. Kidney International, Vol.11, pp.433-442.",
author = "Rudyk, {Svetlana Nikolayevna} and S{\o}gaard, {Erik Gydesen}",
year = "2010",
language = "English",
pages = "312",
booktitle = "8th International Congress on Extremophiles 2010",

}

Rudyk, SN & Søgaard, EG 2010, Alteration of Na+ and K+ ion composition of microbial consortium isolated from oil reservoir at high salinities. i 8th International Congress on Extremophiles 2010: Book of Abstracts. s. 312.

Alteration of Na+ and K+ ion composition of microbial consortium isolated from oil reservoir at high salinities. / Rudyk, Svetlana Nikolayevna; Søgaard, Erik Gydesen.

8th International Congress on Extremophiles 2010: Book of Abstracts. 2010. s. 312.

Publikation: Bidrag til bog/antologi/rapport/konference proceedingKonferenceabstrakt i proceedingForskning

TY - ABST

T1 - Alteration of Na+ and K+ ion composition of microbial consortium isolated from oil reservoir at high salinities

AU - Rudyk, Svetlana Nikolayevna

AU - Søgaard, Erik Gydesen

PY - 2010

Y1 - 2010

N2 - The microbes being injected into the oil layers for the purpose of Microbial Enhanced Oil Recovery (MEOR) undergo the influence of extreme environment of oil reservoir like high salinity, high temperature and high pressure which can suppress their viability and production of the desired by-products such as gases, acids, surfactants and the others. To plan MEOR projects for implementation in the oil reservoirs in the North Sea fields, it needs to take into consideration that the salinity of the formation waters is high, varies in a wide range and depends on the closeness to the salt domes. For the most of bacteria salinity above 50 g/l would have an inhibiting effect. The limits of the optimal conditions for microbial propagation can be overcome through the adaptation processes the bacteria-MEOR candidates go through during laboratory studying. Alteration of the ionic composition is known as playing role in the adaption to salinity [1-3]. The change in the concentration of Na+ and K+ cations in the microbial solution exposed to higher concentrations of sodium chloride was under investigation. The experiment was conducted with the consortium of microbes isolated from the oil-saturated core sample extracted from the depth of 2 km from Dan field at several NaCl concentrations varying from 40 to 70 g/l. The samples of microbial solutions were taken during 11 days. The concentration of Na+ and K+ cations in the solution were measured using ICP/MS spectrometry, Optima 3000 DV. The ion concentrations versus time as a function of salinity were plotted for sodium and potassium separately. In general, for both sodium and potassium ions the lowest concentrations were recorded for salinity of 40 g/l, the highest for 70 g/l with 50 g/l and 60 g/l in between. For Na+ concentration that can be explained solely by salt concentration, but for K+ that effect can be just due to the processes that occurred within microbes. Moreover, the K+-concentration significantly increases and Na+-concentration decreases after 8th day of experiment showing that K+ move out of the cell and Na+ move in. Negative correlation between pH change and K+-concentration was also observed.[1] J. Chr. Skou. (1998). The identification of sodium pump. Nobel Lecture. Bioscience Reports, Vol.18, No.4. [2] Apte S.K., Reddy B.R., Thomas J.(1987). Relationship between Sodium Influx and Salt Tolerance of Nitrogen-Fixing Cyanobacteria. Appl. Environ. Microbiology. 53(8):1934-1939.[3] Sheldon Adler and Donald S. Fraley. (1977). Potassium and intracellular pH. Kidney International, Vol.11, pp.433-442.

AB - The microbes being injected into the oil layers for the purpose of Microbial Enhanced Oil Recovery (MEOR) undergo the influence of extreme environment of oil reservoir like high salinity, high temperature and high pressure which can suppress their viability and production of the desired by-products such as gases, acids, surfactants and the others. To plan MEOR projects for implementation in the oil reservoirs in the North Sea fields, it needs to take into consideration that the salinity of the formation waters is high, varies in a wide range and depends on the closeness to the salt domes. For the most of bacteria salinity above 50 g/l would have an inhibiting effect. The limits of the optimal conditions for microbial propagation can be overcome through the adaptation processes the bacteria-MEOR candidates go through during laboratory studying. Alteration of the ionic composition is known as playing role in the adaption to salinity [1-3]. The change in the concentration of Na+ and K+ cations in the microbial solution exposed to higher concentrations of sodium chloride was under investigation. The experiment was conducted with the consortium of microbes isolated from the oil-saturated core sample extracted from the depth of 2 km from Dan field at several NaCl concentrations varying from 40 to 70 g/l. The samples of microbial solutions were taken during 11 days. The concentration of Na+ and K+ cations in the solution were measured using ICP/MS spectrometry, Optima 3000 DV. The ion concentrations versus time as a function of salinity were plotted for sodium and potassium separately. In general, for both sodium and potassium ions the lowest concentrations were recorded for salinity of 40 g/l, the highest for 70 g/l with 50 g/l and 60 g/l in between. For Na+ concentration that can be explained solely by salt concentration, but for K+ that effect can be just due to the processes that occurred within microbes. Moreover, the K+-concentration significantly increases and Na+-concentration decreases after 8th day of experiment showing that K+ move out of the cell and Na+ move in. Negative correlation between pH change and K+-concentration was also observed.[1] J. Chr. Skou. (1998). The identification of sodium pump. Nobel Lecture. Bioscience Reports, Vol.18, No.4. [2] Apte S.K., Reddy B.R., Thomas J.(1987). Relationship between Sodium Influx and Salt Tolerance of Nitrogen-Fixing Cyanobacteria. Appl. Environ. Microbiology. 53(8):1934-1939.[3] Sheldon Adler and Donald S. Fraley. (1977). Potassium and intracellular pH. Kidney International, Vol.11, pp.433-442.

M3 - Conference abstract in proceeding

SP - 312

BT - 8th International Congress on Extremophiles 2010

ER -

Rudyk SN, Søgaard EG. Alteration of Na+ and K+ ion composition of microbial consortium isolated from oil reservoir at high salinities. I 8th International Congress on Extremophiles 2010: Book of Abstracts. 2010. s. 312