TY - JOUR
T1 - Antibacterial and antioxidant properties of phyto-synthesized silver nanoparticles using Lavandula stoechas extract
AU - Mahmoudi, Reza
AU - Aghaei, Saeed
AU - Salehpour, Zeinab
AU - Mousavizadeh, Ali
AU - Khoramrooz, Seyed Sajjad
AU - Taheripour Sisakht, Marzie
AU - Christiansen, Gunna
AU - Baneshi, Marzieh
AU - Karimi, Bahman
AU - Bardania, Hassan
PY - 2020/2/1
Y1 - 2020/2/1
N2 - Due to environmentally friendly and cost- effective issues, biological methods for silver nanoparticles (AgNPs) synthesis are advantageous over chemical and physical ones. In this study, AgNPs synthesized using Lavandula stoechas extract as a reductant and its antioxidant capacity, antibacterial property and cytotoxicity effect were investigated. The phyto-synthesized AgNPs were characterized using various analyses such as transmission electron microscopy (TEM), scanning electron microscopy (SEM), x-ray diffraction (XRD) as well as Fourier transform infrared (FT-IR). The prepared nanoparticles were spherical on shape with the size about 20–50 nm. Antibacterial studies through agar disk diffusion method confirmed the antibacterial potential of phyto-synthesized AgNPs toward two clinical Staphylococus aureus and Pseudomonas aeruginosa bacteria, although MTT assay demonstrated that S. aureus (MIC = 125 μg/ml) was more susceptible to AgNPs than P. aeruginosa (MIC = 250 μg/ml). Moreover, the cytotoxicity assay of phyto-synthezied AgNPs showed a low cytotoxic effect on RAW264 cell line at 62.5 μg/ml as an effective concentration. Also the considerable antioxidant capacity of the AgNPs confirmed through DPPH assay. Great antibacterial and antioxidant properties along with biocompatibility make the suggested phyto-synthesized AgNPs a great candidate for different biomedical applications including wound healing.
AB - Due to environmentally friendly and cost- effective issues, biological methods for silver nanoparticles (AgNPs) synthesis are advantageous over chemical and physical ones. In this study, AgNPs synthesized using Lavandula stoechas extract as a reductant and its antioxidant capacity, antibacterial property and cytotoxicity effect were investigated. The phyto-synthesized AgNPs were characterized using various analyses such as transmission electron microscopy (TEM), scanning electron microscopy (SEM), x-ray diffraction (XRD) as well as Fourier transform infrared (FT-IR). The prepared nanoparticles were spherical on shape with the size about 20–50 nm. Antibacterial studies through agar disk diffusion method confirmed the antibacterial potential of phyto-synthesized AgNPs toward two clinical Staphylococus aureus and Pseudomonas aeruginosa bacteria, although MTT assay demonstrated that S. aureus (MIC = 125 μg/ml) was more susceptible to AgNPs than P. aeruginosa (MIC = 250 μg/ml). Moreover, the cytotoxicity assay of phyto-synthezied AgNPs showed a low cytotoxic effect on RAW264 cell line at 62.5 μg/ml as an effective concentration. Also the considerable antioxidant capacity of the AgNPs confirmed through DPPH assay. Great antibacterial and antioxidant properties along with biocompatibility make the suggested phyto-synthesized AgNPs a great candidate for different biomedical applications including wound healing.
KW - AgNPs
KW - Lavandula stoechas
KW - antibacterial activity
KW - antioxidant property
KW - green synthesis
UR - http://www.scopus.com/inward/record.url?scp=85076720428&partnerID=8YFLogxK
U2 - 10.1002/aoc.5394
DO - 10.1002/aoc.5394
M3 - Journal article
SN - 0268-2605
VL - 34
JO - Applied Organometallic Chemistry
JF - Applied Organometallic Chemistry
IS - 2
M1 - e5394
ER -