Are commercial stocks of biological control agents genetically depauperate? – A case study on the pirate bug Orius majusculus Reuter

Louise Brink Rasmussen, Kim Jensen, Jesper Givskov Sørensen, Elsa Sverrisdóttir, Kåre Lehmann Nielsen, Johannes Overgaard, Martin Holmstrup, Torsten Nygaard Kristensen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Predatory arthropods are increasingly used in biological control of insect pests. For this purpose, control agents are produced commercially in large quantities for release in crops. The production stocks, however, may have undergone numerous population bottlenecks and may have been exposed to artificial selection pressures in the production facilities. Accordingly, commercial populations may be experiencing loss of genetic variation through inbreeding and genetic drift, which may reduce fitness and biocontrol efficiency. In the present study we investigated whether populations of the pirate bug Orius majusculus (Reuter) purchased from three European companies differed in a range of performance traits including predation rate, starvation tolerance, body size, locomotor activity, and heat tolerance. Furthermore, we crossed all populations pairwise and tested whether outcrossed F2 hybrid offspring had increased performance compared to the parental populations, as would be expected if they were genetically distinct and depauperate. Transcriptome sequencing (RNA-seq) revealed similar overall levels of genetic variation among commercial populations, but also evidence for genetic differentiation. Generally, females performed better across phenotypic traits than males. F2 hybrid offspring differed from parental populations in a highly trait- and sex specific manner. Although F2 hybrids performed better than parental populations in some traits, the results of the present study do not provide conclusive evidence that crossing of different commercial stock populations of O. majusculus improve the genetic quality and performance of this species as a biological control agent.

OriginalsprogEngelsk
TidsskriftBiological Control
Vol/bind127
Sider (fra-til)31-38
Antal sider8
ISSN1049-9644
DOI
StatusUdgivet - 1 dec. 2018

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Orius
biological control agents
case studies
genetic variation
biological control
predatory arthropods
artificial selection
genetic drift
transcriptomics
heat tolerance
insect pests
inbreeding
locomotion
starvation
body size
RNA
predation

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abstract = "Predatory arthropods are increasingly used in biological control of insect pests. For this purpose, control agents are produced commercially in large quantities for release in crops. The production stocks, however, may have undergone numerous population bottlenecks and may have been exposed to artificial selection pressures in the production facilities. Accordingly, commercial populations may be experiencing loss of genetic variation through inbreeding and genetic drift, which may reduce fitness and biocontrol efficiency. In the present study we investigated whether populations of the pirate bug Orius majusculus (Reuter) purchased from three European companies differed in a range of performance traits including predation rate, starvation tolerance, body size, locomotor activity, and heat tolerance. Furthermore, we crossed all populations pairwise and tested whether outcrossed F2 hybrid offspring had increased performance compared to the parental populations, as would be expected if they were genetically distinct and depauperate. Transcriptome sequencing (RNA-seq) revealed similar overall levels of genetic variation among commercial populations, but also evidence for genetic differentiation. Generally, females performed better across phenotypic traits than males. F2 hybrid offspring differed from parental populations in a highly trait- and sex specific manner. Although F2 hybrids performed better than parental populations in some traits, the results of the present study do not provide conclusive evidence that crossing of different commercial stock populations of O. majusculus improve the genetic quality and performance of this species as a biological control agent.",
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Are commercial stocks of biological control agents genetically depauperate? – A case study on the pirate bug Orius majusculus Reuter. / Rasmussen, Louise Brink; Jensen, Kim; Sørensen, Jesper Givskov; Sverrisdóttir, Elsa; Nielsen, Kåre Lehmann; Overgaard, Johannes; Holmstrup, Martin; Kristensen, Torsten Nygaard.

I: Biological Control, Bind 127, 01.12.2018, s. 31-38.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Are commercial stocks of biological control agents genetically depauperate? – A case study on the pirate bug Orius majusculus Reuter

AU - Rasmussen, Louise Brink

AU - Jensen, Kim

AU - Sørensen, Jesper Givskov

AU - Sverrisdóttir, Elsa

AU - Nielsen, Kåre Lehmann

AU - Overgaard, Johannes

AU - Holmstrup, Martin

AU - Kristensen, Torsten Nygaard

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Predatory arthropods are increasingly used in biological control of insect pests. For this purpose, control agents are produced commercially in large quantities for release in crops. The production stocks, however, may have undergone numerous population bottlenecks and may have been exposed to artificial selection pressures in the production facilities. Accordingly, commercial populations may be experiencing loss of genetic variation through inbreeding and genetic drift, which may reduce fitness and biocontrol efficiency. In the present study we investigated whether populations of the pirate bug Orius majusculus (Reuter) purchased from three European companies differed in a range of performance traits including predation rate, starvation tolerance, body size, locomotor activity, and heat tolerance. Furthermore, we crossed all populations pairwise and tested whether outcrossed F2 hybrid offspring had increased performance compared to the parental populations, as would be expected if they were genetically distinct and depauperate. Transcriptome sequencing (RNA-seq) revealed similar overall levels of genetic variation among commercial populations, but also evidence for genetic differentiation. Generally, females performed better across phenotypic traits than males. F2 hybrid offspring differed from parental populations in a highly trait- and sex specific manner. Although F2 hybrids performed better than parental populations in some traits, the results of the present study do not provide conclusive evidence that crossing of different commercial stock populations of O. majusculus improve the genetic quality and performance of this species as a biological control agent.

AB - Predatory arthropods are increasingly used in biological control of insect pests. For this purpose, control agents are produced commercially in large quantities for release in crops. The production stocks, however, may have undergone numerous population bottlenecks and may have been exposed to artificial selection pressures in the production facilities. Accordingly, commercial populations may be experiencing loss of genetic variation through inbreeding and genetic drift, which may reduce fitness and biocontrol efficiency. In the present study we investigated whether populations of the pirate bug Orius majusculus (Reuter) purchased from three European companies differed in a range of performance traits including predation rate, starvation tolerance, body size, locomotor activity, and heat tolerance. Furthermore, we crossed all populations pairwise and tested whether outcrossed F2 hybrid offspring had increased performance compared to the parental populations, as would be expected if they were genetically distinct and depauperate. Transcriptome sequencing (RNA-seq) revealed similar overall levels of genetic variation among commercial populations, but also evidence for genetic differentiation. Generally, females performed better across phenotypic traits than males. F2 hybrid offspring differed from parental populations in a highly trait- and sex specific manner. Although F2 hybrids performed better than parental populations in some traits, the results of the present study do not provide conclusive evidence that crossing of different commercial stock populations of O. majusculus improve the genetic quality and performance of this species as a biological control agent.

KW - Biological control agents

KW - Cross-breeding

KW - Heterosis

KW - Orius majusculus

KW - Predation rate

KW - Stress tolerance

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U2 - 10.1016/j.biocontrol.2018.08.016

DO - 10.1016/j.biocontrol.2018.08.016

M3 - Journal article

VL - 127

SP - 31

EP - 38

JO - Biological Control

JF - Biological Control

SN - 1049-9644

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