Cold acclimation, deacclimation and memory in Arabidopsis

Dirk K Hincha, Stephanie Schaarschmidt, Axel Fischer, Alexander Erban, Majken Pagter, Umarah Mubeen, Dirk Walther, Patrick Giavalisco, Joachim Kopka, Heike Sprenger, Ellen Zuther

Publikation: Konferencebidrag uden forlag/tidsskriftKonferenceabstrakt til konferenceForskning

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Resumé

Plants from temperate regions can be cold acclimated by exposure to low, but non-freezing temperatures resulting in improved freezing tolerance. Whereas the molecular and metabolic basis of cold acclimation has been investigated in detail, little is known about the molecular regulation of deacclimation of cold acclimated plants under warm conditions. Transcript profiling identified genes encoding transcription factors that were transiently induced during the first 24 h of deacclimation, constituting potential regulators determining the rate of loss of freezing tolerance under these conditions. We are currently characterizing the mode of action of the first candidates. In addition, after cold acclimation at 4°C and a deacclimation phase at 20°C, a second treatment at 4°C improved the freezing tolerance of Arabidopsis compared to plants that had only received one cold treatment. This indicates that the plants remembered the first cold treatment and reacted more efficiently to the second cold acclimation. To identify transcripts, lipids and metabolites related to this cold memory, we used Illumina-based RNA-Seq, LC-MS-based lipidomics and GC-MS-based metabolite profiling. We are currently analyzing candidate genes with the aim to identify regulators of cold memory in Arabidopsis.


OriginalsprogEngelsk
Publikationsdato3 jul. 2018
StatusUdgivet - 3 jul. 2018
BegivenhedSEB Florence 2018 - , Italien
Varighed: 3 jul. 20186 jul. 2018

Konference

KonferenceSEB Florence 2018
LandItalien
Periode03/07/201806/07/2018

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acclimation
Arabidopsis
cold tolerance
metabolites
deacclimation
mechanism of action
genes
transcription factors
RNA
lipids
temperature

Citer dette

K Hincha, D., Schaarschmidt, S., Fischer, A., Erban, A., Pagter, M., Mubeen, U., ... Zuther, E. (2018). Cold acclimation, deacclimation and memory in Arabidopsis. Abstract fra SEB Florence 2018, Italien.
K Hincha, Dirk ; Schaarschmidt, Stephanie ; Fischer, Axel ; Erban, Alexander ; Pagter, Majken ; Mubeen, Umarah ; Walther, Dirk ; Giavalisco, Patrick ; Kopka, Joachim ; Sprenger, Heike ; Zuther, Ellen. / Cold acclimation, deacclimation and memory in Arabidopsis. Abstract fra SEB Florence 2018, Italien.
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title = "Cold acclimation, deacclimation and memory in Arabidopsis",
abstract = "Plants from temperate regions can be cold acclimated by exposure to low, but non-freezing temperatures resulting in improved freezing tolerance. Whereas the molecular and metabolic basis of cold acclimation has been investigated in detail, little is known about the molecular regulation of deacclimation of cold acclimated plants under warm conditions. Transcript profiling identified genes encoding transcription factors that were transiently induced during the first 24 h of deacclimation, constituting potential regulators determining the rate of loss of freezing tolerance under these conditions. We are currently characterizing the mode of action of the first candidates. In addition, after cold acclimation at 4°C and a deacclimation phase at 20°C, a second treatment at 4°C improved the freezing tolerance of Arabidopsis compared to plants that had only received one cold treatment. This indicates that the plants remembered the first cold treatment and reacted more efficiently to the second cold acclimation. To identify transcripts, lipids and metabolites related to this cold memory, we used Illumina-based RNA-Seq, LC-MS-based lipidomics and GC-MS-based metabolite profiling. We are currently analyzing candidate genes with the aim to identify regulators of cold memory in Arabidopsis.",
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K Hincha, D, Schaarschmidt, S, Fischer, A, Erban, A, Pagter, M, Mubeen, U, Walther, D, Giavalisco, P, Kopka, J, Sprenger, H & Zuther, E 2018, 'Cold acclimation, deacclimation and memory in Arabidopsis' SEB Florence 2018, Italien, 03/07/2018 - 06/07/2018, .

Cold acclimation, deacclimation and memory in Arabidopsis. / K Hincha, Dirk; Schaarschmidt, Stephanie; Fischer, Axel; Erban, Alexander; Pagter, Majken; Mubeen, Umarah ; Walther, Dirk ; Giavalisco, Patrick ; Kopka, Joachim ; Sprenger, Heike; Zuther, Ellen.

2018. Abstract fra SEB Florence 2018, Italien.

Publikation: Konferencebidrag uden forlag/tidsskriftKonferenceabstrakt til konferenceForskning

TY - ABST

T1 - Cold acclimation, deacclimation and memory in Arabidopsis

AU - K Hincha, Dirk

AU - Schaarschmidt, Stephanie

AU - Fischer, Axel

AU - Erban, Alexander

AU - Pagter, Majken

AU - Mubeen, Umarah

AU - Walther, Dirk

AU - Giavalisco, Patrick

AU - Kopka, Joachim

AU - Sprenger, Heike

AU - Zuther, Ellen

PY - 2018/7/3

Y1 - 2018/7/3

N2 - Plants from temperate regions can be cold acclimated by exposure to low, but non-freezing temperatures resulting in improved freezing tolerance. Whereas the molecular and metabolic basis of cold acclimation has been investigated in detail, little is known about the molecular regulation of deacclimation of cold acclimated plants under warm conditions. Transcript profiling identified genes encoding transcription factors that were transiently induced during the first 24 h of deacclimation, constituting potential regulators determining the rate of loss of freezing tolerance under these conditions. We are currently characterizing the mode of action of the first candidates. In addition, after cold acclimation at 4°C and a deacclimation phase at 20°C, a second treatment at 4°C improved the freezing tolerance of Arabidopsis compared to plants that had only received one cold treatment. This indicates that the plants remembered the first cold treatment and reacted more efficiently to the second cold acclimation. To identify transcripts, lipids and metabolites related to this cold memory, we used Illumina-based RNA-Seq, LC-MS-based lipidomics and GC-MS-based metabolite profiling. We are currently analyzing candidate genes with the aim to identify regulators of cold memory in Arabidopsis.

AB - Plants from temperate regions can be cold acclimated by exposure to low, but non-freezing temperatures resulting in improved freezing tolerance. Whereas the molecular and metabolic basis of cold acclimation has been investigated in detail, little is known about the molecular regulation of deacclimation of cold acclimated plants under warm conditions. Transcript profiling identified genes encoding transcription factors that were transiently induced during the first 24 h of deacclimation, constituting potential regulators determining the rate of loss of freezing tolerance under these conditions. We are currently characterizing the mode of action of the first candidates. In addition, after cold acclimation at 4°C and a deacclimation phase at 20°C, a second treatment at 4°C improved the freezing tolerance of Arabidopsis compared to plants that had only received one cold treatment. This indicates that the plants remembered the first cold treatment and reacted more efficiently to the second cold acclimation. To identify transcripts, lipids and metabolites related to this cold memory, we used Illumina-based RNA-Seq, LC-MS-based lipidomics and GC-MS-based metabolite profiling. We are currently analyzing candidate genes with the aim to identify regulators of cold memory in Arabidopsis.

M3 - Conference abstract for conference

ER -

K Hincha D, Schaarschmidt S, Fischer A, Erban A, Pagter M, Mubeen U et al. Cold acclimation, deacclimation and memory in Arabidopsis. 2018. Abstract fra SEB Florence 2018, Italien.