Abstract
Inhibitory activity in extract from human blood granulocytes was tested on granulocyte-macrophage colony formation in vitro. The inhibition depended on the type of serum used. With mouse BMC and FCS in the cultures, extract corresponding to 2.5 X 10(4) granulocytes/ml reduced the colony number by 35%, and extract from 2 X 10(5) cells caused maximal inhibition (80-90%). With HS and mouse BMC the colony number was reduced by only 11-12%, but stronger inhibition (55%) was observed when the serum concentration was reduced. With both types of sera the total cell number per culture plate was reduced relatively more than the colony number. Human GM-CFC were as sensitive as mouse GM-CFC, and extract from CML granulocytes inhibited less (p less than 0.01) than extract from normal cells. Biochemical studies indicated that the inhibitor is a protein with a molecular weight of 30-60,000. Lactoferrin, a putative inhibitor of CSF production, did not inhibit spontaneous or CSF-induced colony formation in these studies.
Originalsprog | Engelsk |
---|---|
Tidsskrift | European Journal of Haematology |
Vol/bind | 38 |
Udgave nummer | 4 |
Sider (fra-til) | 318-26 |
Antal sider | 9 |
ISSN | 0902-4441 |
Status | Udgivet - 1987 |
Udgivet eksternt | Ja |
Emneord
- Animals
- Cell Extracts
- Cell Survival
- Chromatography, Gel
- Colony-Forming Units Assay
- Female
- Granulocytes
- Humans
- Lactoferrin
- Leukemia, Myeloid
- Macrophages
- Male
- Mice
- Mice, Inbred Strains
- Proteins