Comparative studies of the secretome of fungus-growing ants

Tore Linde, Morten Nedergaard Grell, Morten Schiøtt, Jacobus Jan Boomsma, Lene Lange

Publikation: Bidrag til tidsskriftKonferenceabstrakt i tidsskriftForskning

Resumé


OriginalsprogEngelsk
TidsskriftFungal Genetics Reports
Vol/bind56 (Suppl)
Sider (fra-til)426
ISSN1941-4757
StatusUdgivet - 2009
BegivenhedThe 25th Fungal Genetics Conference at Asilomar - Pacific Grove, USA
Varighed: 17 mar. 200922 mar. 2009

Konference

KonferenceThe 25th Fungal Genetics Conference at Asilomar
LandUSA
ByPacific Grove
Periode17/03/200922/03/2009

Fingerprint

Leucoagaricus
Formicidae
fungi
proteins
cDNA libraries
transposons
trapping
Acromyrmex echinatior
enzymes
antimicrobial proteins
biomass
symbiosis
leaves
nests
screening
nucleotide sequences
nutrients
methodology

Citer dette

Linde, T., Grell, M. N., Schiøtt, M., Boomsma, J. J., & Lange, L. (2009). Comparative studies of the secretome of fungus-growing ants. Fungal Genetics Reports, 56 (Suppl), 426.
Linde, Tore ; Grell, Morten Nedergaard ; Schiøtt, Morten ; Boomsma, Jacobus Jan ; Lange, Lene. / Comparative studies of the secretome of fungus-growing ants. I: Fungal Genetics Reports. 2009 ; Bind 56 (Suppl). s. 426.
@article{91294d50964011de802f000ea68e967b,
title = "Comparative studies of the secretome of fungus-growing ants",
abstract = "Leafcutter ants of the species Acromyrmex echinatior live in symbiosis with the fungus Leucoagaricus gongylophorus. The ants harvest fragments of leaves and carry them to the nest where they place the material on the fungal colony. The fungus secretes a wide array of proteins to degrade the leaves into nutrients that the ants can feed on. The focus of this study is to discover, characterize and compare the secreted proteins. In order to do so cDNA libraries are constructed from mRNA extracted from the fungus material. The most efficient technology to screen cDNA libraries selectively for secreted and membrane-bound proteins is the TAST (Transposon Assisted Signal Trapping) technology (Becker et al. 2004,Microbiol Methods 57, 123-133). The TAST screening will give a list of full length gene sequences encoding secreted proteins. The main part of the secretome will consist of biomass degrading enzymes, but also antimicrobial proteins will probably be in the secretome. To date only a few enzymes from the Leucoagaricus secretome have been identified. We expect to discover novel proteins and to gain a better understanding of the biodegrading pathways of Leucoagaricus. Ultimately, this work may identify enzymes that can be used in biomass conversion processes. (Poster # 426)",
keywords = "Symbiotic interactions, Fungus-host interactions, Gene discovery, Extracellular proteins",
author = "Tore Linde and Grell, {Morten Nedergaard} and Morten Schi{\o}tt and Boomsma, {Jacobus Jan} and Lene Lange",
year = "2009",
language = "English",
volume = "56 (Suppl)",
pages = "426",
journal = "Fungal Genetics Reports",
issn = "1941-4757",
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Linde, T, Grell, MN, Schiøtt, M, Boomsma, JJ & Lange, L 2009, 'Comparative studies of the secretome of fungus-growing ants', Fungal Genetics Reports, bind 56 (Suppl), s. 426.

Comparative studies of the secretome of fungus-growing ants. / Linde, Tore; Grell, Morten Nedergaard; Schiøtt, Morten; Boomsma, Jacobus Jan; Lange, Lene.

I: Fungal Genetics Reports, Bind 56 (Suppl), 2009, s. 426.

Publikation: Bidrag til tidsskriftKonferenceabstrakt i tidsskriftForskning

TY - ABST

T1 - Comparative studies of the secretome of fungus-growing ants

AU - Linde, Tore

AU - Grell, Morten Nedergaard

AU - Schiøtt, Morten

AU - Boomsma, Jacobus Jan

AU - Lange, Lene

PY - 2009

Y1 - 2009

N2 - Leafcutter ants of the species Acromyrmex echinatior live in symbiosis with the fungus Leucoagaricus gongylophorus. The ants harvest fragments of leaves and carry them to the nest where they place the material on the fungal colony. The fungus secretes a wide array of proteins to degrade the leaves into nutrients that the ants can feed on. The focus of this study is to discover, characterize and compare the secreted proteins. In order to do so cDNA libraries are constructed from mRNA extracted from the fungus material. The most efficient technology to screen cDNA libraries selectively for secreted and membrane-bound proteins is the TAST (Transposon Assisted Signal Trapping) technology (Becker et al. 2004,Microbiol Methods 57, 123-133). The TAST screening will give a list of full length gene sequences encoding secreted proteins. The main part of the secretome will consist of biomass degrading enzymes, but also antimicrobial proteins will probably be in the secretome. To date only a few enzymes from the Leucoagaricus secretome have been identified. We expect to discover novel proteins and to gain a better understanding of the biodegrading pathways of Leucoagaricus. Ultimately, this work may identify enzymes that can be used in biomass conversion processes. (Poster # 426)

AB - Leafcutter ants of the species Acromyrmex echinatior live in symbiosis with the fungus Leucoagaricus gongylophorus. The ants harvest fragments of leaves and carry them to the nest where they place the material on the fungal colony. The fungus secretes a wide array of proteins to degrade the leaves into nutrients that the ants can feed on. The focus of this study is to discover, characterize and compare the secreted proteins. In order to do so cDNA libraries are constructed from mRNA extracted from the fungus material. The most efficient technology to screen cDNA libraries selectively for secreted and membrane-bound proteins is the TAST (Transposon Assisted Signal Trapping) technology (Becker et al. 2004,Microbiol Methods 57, 123-133). The TAST screening will give a list of full length gene sequences encoding secreted proteins. The main part of the secretome will consist of biomass degrading enzymes, but also antimicrobial proteins will probably be in the secretome. To date only a few enzymes from the Leucoagaricus secretome have been identified. We expect to discover novel proteins and to gain a better understanding of the biodegrading pathways of Leucoagaricus. Ultimately, this work may identify enzymes that can be used in biomass conversion processes. (Poster # 426)

KW - Symbiotic interactions

KW - Fungus-host interactions

KW - Gene discovery

KW - Extracellular proteins

M3 - Conference abstract in journal

VL - 56 (Suppl)

SP - 426

JO - Fungal Genetics Reports

JF - Fungal Genetics Reports

SN - 1941-4757

ER -

Linde T, Grell MN, Schiøtt M, Boomsma JJ, Lange L. Comparative studies of the secretome of fungus-growing ants. Fungal Genetics Reports. 2009;56 (Suppl):426.