TY - JOUR
T1 - Controlling Aspergillus niger morphology in a low shear-force environment in a rocking-motion bioreactor
AU - Kheirkhah, Tolue
AU - Neubauer, Peter
AU - Junne, Stefan
PY - 2023/6
Y1 - 2023/6
N2 - The filamentous fungus Aspergillus niger is an important production host in biotechnology. Shear force regimes are one of the key factors that affect macromorphology and product yield. While morphology changes under intensive agitation have been widely investigated, studies at a low shear force regime independently from oxygen limitation has remained a challenge. Therefore, in this study, a 2-dimensional rocking-motion bioreactor is used as an alternative platform for studying the macromorphology under a low shear force regime, but sufficient supply of dissolved oxygen. Talcum macroparticles were added at different concentrations to control the development of a certain macromorphology. Results showed that 0.25% and 1% (ww−1) of talcum led to a mixture of dispersed mycelia and loose clumps, similar to what is obtained in lab-scale stirred tank reactors. At lower talcum concentrations, distinct pellet formation was observed. Quantitative analysis of pellets showed that with 0.05% of talcum, 95% of the pellets had a diameter smaller than 850 µm after 36 h. In case of 0.1% of talcum, 94% ( ± 5.0%) of the pellets had a diameter below 650 µm.The presented approach makes it possible to achieve a certain morphology as, for example, observed in large scale cultivations to study the consequences for product synthesis.
AB - The filamentous fungus Aspergillus niger is an important production host in biotechnology. Shear force regimes are one of the key factors that affect macromorphology and product yield. While morphology changes under intensive agitation have been widely investigated, studies at a low shear force regime independently from oxygen limitation has remained a challenge. Therefore, in this study, a 2-dimensional rocking-motion bioreactor is used as an alternative platform for studying the macromorphology under a low shear force regime, but sufficient supply of dissolved oxygen. Talcum macroparticles were added at different concentrations to control the development of a certain macromorphology. Results showed that 0.25% and 1% (ww−1) of talcum led to a mixture of dispersed mycelia and loose clumps, similar to what is obtained in lab-scale stirred tank reactors. At lower talcum concentrations, distinct pellet formation was observed. Quantitative analysis of pellets showed that with 0.05% of talcum, 95% of the pellets had a diameter smaller than 850 µm after 36 h. In case of 0.1% of talcum, 94% ( ± 5.0%) of the pellets had a diameter below 650 µm.The presented approach makes it possible to achieve a certain morphology as, for example, observed in large scale cultivations to study the consequences for product synthesis.
KW - Aspergillus niger
KW - Morphology control
KW - Pellet formation
KW - Rocking-motion bioreactor
KW - Shear stress
KW - Talcum microparticles
UR - http://www.scopus.com/inward/record.url?scp=85151018288&partnerID=8YFLogxK
U2 - 10.1016/j.bej.2023.108905
DO - 10.1016/j.bej.2023.108905
M3 - Journal article
SN - 1369-703X
VL - 195
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
M1 - 108905
ER -