Corrigendum: Strategies for Efficient Gene Editing in Protoplasts of Solanum tuberosum Theme: Determining gRNA Efficiency Design by Utilizing Protoplast (Research) (Frontiers in Genome Editing, (2022), 3, (795644), 10.3389/fgeed.2021.795644)

In the original article, there were various errors present throughout the main text. These errors have been corrected in the original article. Additionally, Figure 1 and Table 1 have been updated. The updated figure and table are shown below. The Funding statement has also been updated and is shown below. Glucan Water Dikinase (GWD) 1—structure and full allelic sequence of gRNA target regions. Overall gene structure with exons (boxes) and the area containing carbohydrate-binding module (CBM) depicted above. Left: the nucleotide sequence of exon 1 and introns. Right: exon 24 and 25 including introns. Exons are depicted in capital letters with the amino acid sequence indicated. Small nucleotide polymorphisms (SNPs) from cultivars included in the SPUD database are marked with red, and SNPs found in Saturna are underlined. Grey arrows designate gRNAs (gA, gB, gC, gD, gE, gI, gJ, gK, gL, and gM) with PAM sites marked in bold. Red arrows designate diagnostic IDAA PCR primers. The “CFATC” region, containing cysteine’s hypothesized to be involved in inter or intra-di-sulfide bond formation and thus in putative redox-state modulation of GWD activity is marked with bold. The active site histidine residue is also marked with bold. gRNAs and diagnostic IDAA primers for each of the four target regions. Scores and first selection of gRNAs were obtained by feeding ca 1 kb regions to the in silico prediction servers CHOPCHOP (http://chopchop.cbu.uib.no/), CRISPRater (https://crispr.cos.uniheidelberg.de/) and SSC (http://crispr.dfci.harvard.edu/SSC/).