TY - JOUR
T1 - Data for automated, high-throughput microscopy analysis of intracellular bacterial colonies using spot detection
AU - Ernstsen, Christina L.
AU - Login, Frédéric H.
AU - Jensen, Helene H.
AU - Nørregaard, Rikke
AU - Møller-Jensen, Jakob
AU - Nejsum, Lene N.
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Quantification of intracellular bacterial colonies is useful in strategies directed against bacterial attachment, subsequent cellular invasion and intracellular proliferation. An automated, high-throughput microscopy-method was established to quantify the number and size of intracellular bacterial colonies in infected host cells (Detection and quantification of intracellular bacterial colonies by automated, high-throughput microscopy, Ernstsen et al., 2017 [1]). The infected cells were imaged with a 10× objective and number of intracellular bacterial colonies, their size distribution and the number of cell nuclei were automatically quantified using a spot detection-tool. The spot detection-output was exported to Excel, where data analysis was performed. In this article, micrographs and spot detection data are made available to facilitate implementation of the method.
AB - Quantification of intracellular bacterial colonies is useful in strategies directed against bacterial attachment, subsequent cellular invasion and intracellular proliferation. An automated, high-throughput microscopy-method was established to quantify the number and size of intracellular bacterial colonies in infected host cells (Detection and quantification of intracellular bacterial colonies by automated, high-throughput microscopy, Ernstsen et al., 2017 [1]). The infected cells were imaged with a 10× objective and number of intracellular bacterial colonies, their size distribution and the number of cell nuclei were automatically quantified using a spot detection-tool. The spot detection-output was exported to Excel, where data analysis was performed. In this article, micrographs and spot detection data are made available to facilitate implementation of the method.
KW - High-throughput imaging
KW - Intracellular bacterial colonies
KW - Invasive bacteria
KW - Spot detection
KW - UPEC
KW - Uropathogenic E. coli
UR - http://www.scopus.com/inward/record.url?scp=85028733601&partnerID=8YFLogxK
U2 - 10.1016/j.dib.2017.08.027
DO - 10.1016/j.dib.2017.08.027
M3 - Journal article
AN - SCOPUS:85028733601
SN - 2352-3409
VL - 14
SP - 643
EP - 647
JO - Data in Brief
JF - Data in Brief
ER -