Dechloromonas: to be or not to be a PAO? That is the question!

Francesca Petriglieri; Caitlin Margaret Singleton; Miriam Peces Gomez; Jette Fischer Petersen; Marta Anna Nierychlo; Per Halkjær Nielsen

Dechloromonas: to be or not to be a PAO? That is the question!

Francesca Petriglieri, Caitlin Margaret Singleton, Miriam Peces Gomez, Jette Fischer Petersen, Marta Anna Nierychlo, Per Halkjær Nielsen

Publikation: Konferencebidrag uden forlag/tidsskrift › Poster › Forskning

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Abstract

Members of the genus Dechloromonas are often abundant in EBPR plants worldwide and have long been considered putative PAOs, as intracellular poly-P has been identified with traditional staining methods. This study applied metagenomics to determine the metabolic potential and Raman microspectroscopy to verify and define the levels and dynamics of important storage polymers in FISH-defined Dechloromonas cells. A 99.2% complete metagenome-assembled genome, retrieved from Danish EBPR plants, showed the potential for poly-P, glycogen, and PHA accumulation. Dechloromonas cells exhibited in situ a Ca. Accumulibacter-like phenotype with dynamic levels of poly-P and PHA during feast-famine cycling. Quantitative Raman-FISH analysis of single cells from full-scale activated sludge plants confirmed Dechloromonas to be an important PAO.

Originalsprog	Engelsk
Publikationsdato	18 nov. 2019
Status	Udgivet - 18 nov. 2019
Begivenhed	8th IWA Microbial Ecology and Water Engineering Specialist Conference - Hiroshima, Japan Varighed: 17 nov. 2019 → 20 nov. 2019 https://iwa-network.org/events/microbial-ecology-and-water-engineering-specialist-group-conference/

Konference

Konference	8th IWA Microbial Ecology and Water Engineering Specialist Conference
Land/Område	Japan
By	Hiroshima
Periode	17/11/2019 → 20/11/2019
Internetadresse	https://iwa-network.org/events/microbial-ecology-and-water-engineering-specialist-group-conference/

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Dechloromonas: to be or not to be a PAO? That is the question!

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title = "Dechloromonas: to be or not to be a PAO? That is the question!",

abstract = "Members of the genus Dechloromonas are often abundant in EBPR plants worldwide and have long been considered putative PAOs, as intracellular poly-P has been identified with traditional staining methods. This study applied metagenomics to determine the metabolic potential and Raman microspectroscopy to verify and define the levels and dynamics of important storage polymers in FISH-defined Dechloromonas cells. A 99.2% complete metagenome-assembled genome, retrieved from Danish EBPR plants, showed the potential for poly-P, glycogen, and PHA accumulation. Dechloromonas cells exhibited in situ a Ca. Accumulibacter-like phenotype with dynamic levels of poly-P and PHA during feast-famine cycling. Quantitative Raman-FISH analysis of single cells from full-scale activated sludge plants confirmed Dechloromonas to be an important PAO.",

keywords = "Dechloromonas, PAO, Raman-FISH, P mass-balance",

author = "Francesca Petriglieri and Singleton, {Caitlin Margaret} and Gomez, {Miriam Peces} and Petersen, {Jette Fischer} and Nierychlo, {Marta Anna} and Nielsen, {Per Halkj{\ae}r}",

year = "2019",

month = nov,

day = "18",

language = "English",

note = "8th IWA Microbial Ecology and Water Engineering Specialist Conference ; Conference date: 17-11-2019 Through 20-11-2019",

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T1 - Dechloromonas: to be or not to be a PAO? That is the question!

AU - Petriglieri, Francesca

AU - Singleton, Caitlin Margaret

AU - Gomez, Miriam Peces

AU - Petersen, Jette Fischer

AU - Nierychlo, Marta Anna

AU - Nielsen, Per Halkjær

PY - 2019/11/18

Y1 - 2019/11/18

N2 - Members of the genus Dechloromonas are often abundant in EBPR plants worldwide and have long been considered putative PAOs, as intracellular poly-P has been identified with traditional staining methods. This study applied metagenomics to determine the metabolic potential and Raman microspectroscopy to verify and define the levels and dynamics of important storage polymers in FISH-defined Dechloromonas cells. A 99.2% complete metagenome-assembled genome, retrieved from Danish EBPR plants, showed the potential for poly-P, glycogen, and PHA accumulation. Dechloromonas cells exhibited in situ a Ca. Accumulibacter-like phenotype with dynamic levels of poly-P and PHA during feast-famine cycling. Quantitative Raman-FISH analysis of single cells from full-scale activated sludge plants confirmed Dechloromonas to be an important PAO.

AB - Members of the genus Dechloromonas are often abundant in EBPR plants worldwide and have long been considered putative PAOs, as intracellular poly-P has been identified with traditional staining methods. This study applied metagenomics to determine the metabolic potential and Raman microspectroscopy to verify and define the levels and dynamics of important storage polymers in FISH-defined Dechloromonas cells. A 99.2% complete metagenome-assembled genome, retrieved from Danish EBPR plants, showed the potential for poly-P, glycogen, and PHA accumulation. Dechloromonas cells exhibited in situ a Ca. Accumulibacter-like phenotype with dynamic levels of poly-P and PHA during feast-famine cycling. Quantitative Raman-FISH analysis of single cells from full-scale activated sludge plants confirmed Dechloromonas to be an important PAO.

KW - Dechloromonas

KW - PAO

KW - Raman-FISH

KW - P mass-balance

M3 - Poster

T2 - 8th IWA Microbial Ecology and Water Engineering Specialist Conference

Y2 - 17 November 2019 through 20 November 2019

ER -

Dechloromonas: to be or not to be a PAO? That is the question!

Abstract

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