TY - JOUR
T1 - Demonstration of CDX2 is highly antibody dependant
AU - Borrisholt, Martine
AU - Nielsen, Søren
AU - Vyberg, Mogens
PY - 2013/1
Y1 - 2013/1
N2 - CDX2 is a widely used immunohistochemical marker for intestinal differentiation in neoplasms. In the Nordic Immunohistochemical Quality Control external quality assessment scheme, only 45% of the laboratories participating in the CDX2 challenge in 2009 produced sufficient staining. A major cause of insufficient staining results appeared to be less successful primary antibody (Ab) clones. To evaluate the Ab performance in a standardized way, a comparative study was carried out.
AB - CDX2 is a widely used immunohistochemical marker for intestinal differentiation in neoplasms. In the Nordic Immunohistochemical Quality Control external quality assessment scheme, only 45% of the laboratories participating in the CDX2 challenge in 2009 produced sufficient staining. A major cause of insufficient staining results appeared to be less successful primary antibody (Ab) clones. To evaluate the Ab performance in a standardized way, a comparative study was carried out.
KW - Adenocarcinoma
KW - Antibodies, Monoclonal
KW - Carcinoma, Squamous Cell
KW - Cell Transformation, Neoplastic
KW - Denmark
KW - Diagnosis, Differential
KW - Homeodomain Proteins
KW - Humans
KW - Immunohistochemistry
KW - Intestinal Neoplasms
KW - Lung Neoplasms
KW - Quality Assurance, Health Care
KW - Quality Control
KW - Quality Improvement
KW - Sensitivity and Specificity
KW - Tissue Array Analysis
KW - Tumor Markers, Biological
U2 - 10.1097/PAI.0b013e318257f8aa
DO - 10.1097/PAI.0b013e318257f8aa
M3 - Journal article
C2 - 22595949
SN - 1541-2016
VL - 21
SP - 64
EP - 72
JO - Applied Immunohistochemistry & Molecular Morphology
JF - Applied Immunohistochemistry & Molecular Morphology
IS - 1
ER -