Development of bipolar prosthecae by candidate phylum Acetothermia bacteria

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskning

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Resumé

Members of the candidate phylum Acetothermia are globally distributed and detected in various habitats. However, little is known about their physiology and ecological importance. We recently discovered an operational taxonomic unit (OTU) belonging to Acetothermia that were at high abundance in four full-scale anaerobic digesters by 16S rRNA gene amplicon sequencing. Using differential coverage binning of metagenomes and scaffolding with long nanopore reads we were able to close the first genome from this phylum. Genome annotation and metabolic reconstruction suggested an anaerobic chemoheterotrophic lifestyle in which the bacterium obtain energy and carbon via fermentation of peptides, amino acids, and simple sugars to acetate, formate, and hydrogen. Fluorescence in situ hybridization combined with confocal laser scanning microscopy, Raman microspectroscopy and atomic force microscopy revealed an unusual morphology composed of a central rod-shaped cell with bipolar prosthecae. We hypothesize that these prosthecae allow for increased nutrient uptake by greatly expanding the cell surface area, providing a competitive advantage under nutrient-limited conditions.
OriginalsprogEngelsk
Publikationsdato16 aug. 2018
StatusUdgivet - 16 aug. 2018
Begivenhed17th International Symposium on Microbial Ecology: ISME17 - Leipziger Messe Exhibition and Convention Centre, Leipzig, Tyskland
Varighed: 12 aug. 201817 aug. 2018
Konferencens nummer: 17
https://isme17.isme-microbes.org/

Konference

Konference17th International Symposium on Microbial Ecology
Nummer17
LokationLeipziger Messe Exhibition and Convention Centre
LandTyskland
ByLeipzig
Periode12/08/201817/08/2018
Internetadresse

Citer dette

Hao, L., McIlroy, S. J., Kirkegaard, R. H., Karst, S. M., Fernando, E., Aslan, H., ... Dueholm, M. S. (2018). Development of bipolar prosthecae by candidate phylum Acetothermia bacteria. Poster session præsenteret på 17th International Symposium on Microbial Ecology, Leipzig, Tyskland.
Hao, Liping ; McIlroy, Simon Jon ; Kirkegaard, Rasmus Hansen ; Karst, Søren Michael ; Fernando, Eustace ; Aslan, Hüsnü ; Meyer, Rikke ; Albertsen, Mads ; Nielsen, Per Halkjær ; Dueholm, Morten Simonsen. / Development of bipolar prosthecae by candidate phylum Acetothermia bacteria. Poster session præsenteret på 17th International Symposium on Microbial Ecology, Leipzig, Tyskland.
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title = "Development of bipolar prosthecae by candidate phylum Acetothermia bacteria",
abstract = "Members of the candidate phylum Acetothermia are globally distributed and detected in various habitats. However, little is known about their physiology and ecological importance. We recently discovered an operational taxonomic unit (OTU) belonging to Acetothermia that were at high abundance in four full-scale anaerobic digesters by 16S rRNA gene amplicon sequencing. Using differential coverage binning of metagenomes and scaffolding with long nanopore reads we were able to close the first genome from this phylum. Genome annotation and metabolic reconstruction suggested an anaerobic chemoheterotrophic lifestyle in which the bacterium obtain energy and carbon via fermentation of peptides, amino acids, and simple sugars to acetate, formate, and hydrogen. Fluorescence in situ hybridization combined with confocal laser scanning microscopy, Raman microspectroscopy and atomic force microscopy revealed an unusual morphology composed of a central rod-shaped cell with bipolar prosthecae. We hypothesize that these prosthecae allow for increased nutrient uptake by greatly expanding the cell surface area, providing a competitive advantage under nutrient-limited conditions.",
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Hao, L, McIlroy, SJ, Kirkegaard, RH, Karst, SM, Fernando, E, Aslan, H, Meyer, R, Albertsen, M, Nielsen, PH & Dueholm, MS 2018, 'Development of bipolar prosthecae by candidate phylum Acetothermia bacteria' 17th International Symposium on Microbial Ecology, Leipzig, Tyskland, 12/08/2018 - 17/08/2018, .

Development of bipolar prosthecae by candidate phylum Acetothermia bacteria. / Hao, Liping; McIlroy, Simon Jon; Kirkegaard, Rasmus Hansen; Karst, Søren Michael; Fernando, Eustace; Aslan, Hüsnü; Meyer, Rikke; Albertsen, Mads; Nielsen, Per Halkjær; Dueholm, Morten Simonsen.

2018. Poster session præsenteret på 17th International Symposium on Microbial Ecology, Leipzig, Tyskland.

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskning

TY - CONF

T1 - Development of bipolar prosthecae by candidate phylum Acetothermia bacteria

AU - Hao, Liping

AU - McIlroy, Simon Jon

AU - Kirkegaard, Rasmus Hansen

AU - Karst, Søren Michael

AU - Fernando, Eustace

AU - Aslan, Hüsnü

AU - Meyer, Rikke

AU - Albertsen, Mads

AU - Nielsen, Per Halkjær

AU - Dueholm, Morten Simonsen

PY - 2018/8/16

Y1 - 2018/8/16

N2 - Members of the candidate phylum Acetothermia are globally distributed and detected in various habitats. However, little is known about their physiology and ecological importance. We recently discovered an operational taxonomic unit (OTU) belonging to Acetothermia that were at high abundance in four full-scale anaerobic digesters by 16S rRNA gene amplicon sequencing. Using differential coverage binning of metagenomes and scaffolding with long nanopore reads we were able to close the first genome from this phylum. Genome annotation and metabolic reconstruction suggested an anaerobic chemoheterotrophic lifestyle in which the bacterium obtain energy and carbon via fermentation of peptides, amino acids, and simple sugars to acetate, formate, and hydrogen. Fluorescence in situ hybridization combined with confocal laser scanning microscopy, Raman microspectroscopy and atomic force microscopy revealed an unusual morphology composed of a central rod-shaped cell with bipolar prosthecae. We hypothesize that these prosthecae allow for increased nutrient uptake by greatly expanding the cell surface area, providing a competitive advantage under nutrient-limited conditions.

AB - Members of the candidate phylum Acetothermia are globally distributed and detected in various habitats. However, little is known about their physiology and ecological importance. We recently discovered an operational taxonomic unit (OTU) belonging to Acetothermia that were at high abundance in four full-scale anaerobic digesters by 16S rRNA gene amplicon sequencing. Using differential coverage binning of metagenomes and scaffolding with long nanopore reads we were able to close the first genome from this phylum. Genome annotation and metabolic reconstruction suggested an anaerobic chemoheterotrophic lifestyle in which the bacterium obtain energy and carbon via fermentation of peptides, amino acids, and simple sugars to acetate, formate, and hydrogen. Fluorescence in situ hybridization combined with confocal laser scanning microscopy, Raman microspectroscopy and atomic force microscopy revealed an unusual morphology composed of a central rod-shaped cell with bipolar prosthecae. We hypothesize that these prosthecae allow for increased nutrient uptake by greatly expanding the cell surface area, providing a competitive advantage under nutrient-limited conditions.

M3 - Poster

ER -

Hao L, McIlroy SJ, Kirkegaard RH, Karst SM, Fernando E, Aslan H et al. Development of bipolar prosthecae by candidate phylum Acetothermia bacteria. 2018. Poster session præsenteret på 17th International Symposium on Microbial Ecology, Leipzig, Tyskland.