TY - JOUR
T1 - Early stage inflammation changes in supraspinatus muscle after rotator cuff tear
AU - Stengaard, Kira
AU - Hejbøl, Eva Kildall
AU - Jensen, Peter Toft
AU - Degn, Matilda
AU - Linh Ta, Thi My
AU - Stensballe, Allan
AU - Andersen, Ditte Caroline
AU - Schrøder, Henrik Daa
AU - Lambertsen, Kate Lykke
AU - Frich, Lars Henrik
N1 - Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.
PY - 2022/7
Y1 - 2022/7
N2 - Background: Rotator cuff (RC) tendon tear leads to impaired shoulder function and pain. The supraspinatus (SS) tendon is most often affected, but the biological response of the SS muscle to SS tendon tear is largely unknown. This study aimed to investigate time-dependent muscle inflammation, degeneration, fatty infiltration, and regeneration in experimental SS tear conditions. Methods: Forty-five C57BL/6 mice were subjected to SS tendon tear and allowed to recover for 1, 3, 5, 7, 14, or 28 days. The extent of muscle damage was examined using histologic, flow cytometric, proteomic, and chemiluminescence analyses. Results: We found that muscle inflammation peaked around day 5 with increased monocyte infiltration and increased cytokine levels in the ipsilateral compared to the contralateral SS muscle. Bioinformatics analysis of proteomics on mice that survived 5 days after RC tendon tear revealed upregulated proteins involved in “neutrophil activation involved in immune response” and “extracellular matrix organization,” whereas “skeletal muscle tissue development and contraction” and “respiratory electron transport chain” were among the most downregulated. Histologic analysis of collagen showed increased collagen accumulation and fatty infiltration of the ipsilateral SS over time. Finally, we observed time- and lesion-dependent changes in satellite cell and fibro-adipogenic progenitor populations. Conclusion: Altogether, we demonstrate that the SS muscle shows severe signs of acute inflammation, early degeneration, and fatty infiltration, as well as reduced regenerative potential following SS tendon tear.
AB - Background: Rotator cuff (RC) tendon tear leads to impaired shoulder function and pain. The supraspinatus (SS) tendon is most often affected, but the biological response of the SS muscle to SS tendon tear is largely unknown. This study aimed to investigate time-dependent muscle inflammation, degeneration, fatty infiltration, and regeneration in experimental SS tear conditions. Methods: Forty-five C57BL/6 mice were subjected to SS tendon tear and allowed to recover for 1, 3, 5, 7, 14, or 28 days. The extent of muscle damage was examined using histologic, flow cytometric, proteomic, and chemiluminescence analyses. Results: We found that muscle inflammation peaked around day 5 with increased monocyte infiltration and increased cytokine levels in the ipsilateral compared to the contralateral SS muscle. Bioinformatics analysis of proteomics on mice that survived 5 days after RC tendon tear revealed upregulated proteins involved in “neutrophil activation involved in immune response” and “extracellular matrix organization,” whereas “skeletal muscle tissue development and contraction” and “respiratory electron transport chain” were among the most downregulated. Histologic analysis of collagen showed increased collagen accumulation and fatty infiltration of the ipsilateral SS over time. Finally, we observed time- and lesion-dependent changes in satellite cell and fibro-adipogenic progenitor populations. Conclusion: Altogether, we demonstrate that the SS muscle shows severe signs of acute inflammation, early degeneration, and fatty infiltration, as well as reduced regenerative potential following SS tendon tear.
KW - Adipose Tissue/pathology
KW - Animals
KW - Humans
KW - Inflammation
KW - Mice
KW - Mice, Inbred C57BL
KW - Muscular Atrophy/pathology
KW - Proteomics
KW - Rotator Cuff Injuries
KW - Rotator Cuff/pathology
UR - http://www.scopus.com/inward/record.url?scp=85132454512&partnerID=8YFLogxK
U2 - 10.1016/j.jse.2021.12.046
DO - 10.1016/j.jse.2021.12.046
M3 - Journal article
C2 - 35150831
SN - 1058-2746
VL - 31
SP - 1344
EP - 1356
JO - Journal of Shoulder and Elbow Surgery
JF - Journal of Shoulder and Elbow Surgery
IS - 7
ER -