Effect of environmental pH on sperm kinematic characteristics

Hiva Alipour, Fereshteh Dardmeh, Marianne Charlotte Dissing, H. Ingolf Nielsen

Publikation: Bidrag til bog/antologi/rapport/konference proceedingKonferenceabstrakt i proceedingForskning

Resumé

Semen preparation medium have an important role in assisted reproduction techniques and their composition influences sperm binding and motility.
Some studies have assessed the influence of pH on sperm kinetics. However, no study to date has assessed the effect of environmental pH on subtle differences in the details of the sperm movement (kinematics) of human sperm provided by computerized sperm analysis systems. This study was designed to assess the effect of two different media pH levels on kinematic parameters of the human sperm.
Samples were prepared using the 40%/80% Pureception (Sage, USA) density gradient and resuspended in customized sperm culture media with pH levels of 7.9 and 8.3 (Origio, Denmark). Kinematic parameters of the sperm in both groups were analyzed using the Sperm Class Analyzer (Microptic S.L., Spain) at 0, 6 and 24 hours following the addition of media.
Results of this study illustrated a general insignificant decrease in the ratio of progressively motile and hyperactive sperm after 6 and 24 hours. However a significant difference between the test groups was observed in the curvilinear, straight line and Mean path velocity and Straightness after 6 and 24 hours. Linearity and Wobble showed significant difference after 24 hours.
This study demonstrated a difference in the sperm motion pattern and velocity in different environmental pH levels. Based on these findings, further investigations are required to elucidate knowledge about possible effect of marginal pH changes, affecting the sperm motion characteristics at different stages in the dynamic environment of the female reproductive tract.
OriginalsprogEngelsk
TitelFinal programme, 18th World Congress of In Vitro Ferilization, ISIVF 2015, 27-30 September 2015, Copenhagen, Denmark
ForlagInternational Society of In Vitro Fertilization
Publikationsdatosep. 2015
Sider22, No. P16
StatusUdgivet - sep. 2015
BegivenhedWorld Congress of In Vitro Fertilization, ISIVF - Copenhagen, Danmark
Varighed: 27 sep. 201530 sep. 2015
Konferencens nummer: 18

Konference

KonferenceWorld Congress of In Vitro Fertilization, ISIVF
Nummer18
LandDanmark
ByCopenhagen
Periode27/09/201530/09/2015

Fingerprint

Biomechanical Phenomena
Spermatozoa
Reproductive Techniques
Sperm Motility
Denmark
Semen
Spain
Culture Media

Citer dette

Alipour, H., Dardmeh, F., Dissing, M. C., & Nielsen, H. I. (2015). Effect of environmental pH on sperm kinematic characteristics. I Final programme, 18th World Congress of In Vitro Ferilization, ISIVF 2015, 27-30 September 2015, Copenhagen, Denmark (s. 22, No. P16). International Society of In Vitro Fertilization.
Alipour, Hiva ; Dardmeh, Fereshteh ; Dissing, Marianne Charlotte ; Nielsen, H. Ingolf. / Effect of environmental pH on sperm kinematic characteristics. Final programme, 18th World Congress of In Vitro Ferilization, ISIVF 2015, 27-30 September 2015, Copenhagen, Denmark. International Society of In Vitro Fertilization, 2015. s. 22, No. P16
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abstract = "Semen preparation medium have an important role in assisted reproduction techniques and their composition influences sperm binding and motility.Some studies have assessed the influence of pH on sperm kinetics. However, no study to date has assessed the effect of environmental pH on subtle differences in the details of the sperm movement (kinematics) of human sperm provided by computerized sperm analysis systems. This study was designed to assess the effect of two different media pH levels on kinematic parameters of the human sperm.Samples were prepared using the 40{\%}/80{\%} Pureception (Sage, USA) density gradient and resuspended in customized sperm culture media with pH levels of 7.9 and 8.3 (Origio, Denmark). Kinematic parameters of the sperm in both groups were analyzed using the Sperm Class Analyzer (Microptic S.L., Spain) at 0, 6 and 24 hours following the addition of media.Results of this study illustrated a general insignificant decrease in the ratio of progressively motile and hyperactive sperm after 6 and 24 hours. However a significant difference between the test groups was observed in the curvilinear, straight line and Mean path velocity and Straightness after 6 and 24 hours. Linearity and Wobble showed significant difference after 24 hours.This study demonstrated a difference in the sperm motion pattern and velocity in different environmental pH levels. Based on these findings, further investigations are required to elucidate knowledge about possible effect of marginal pH changes, affecting the sperm motion characteristics at different stages in the dynamic environment of the female reproductive tract.",
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Alipour, H, Dardmeh, F, Dissing, MC & Nielsen, HI 2015, Effect of environmental pH on sperm kinematic characteristics. i Final programme, 18th World Congress of In Vitro Ferilization, ISIVF 2015, 27-30 September 2015, Copenhagen, Denmark. International Society of In Vitro Fertilization, s. 22, No. P16, World Congress of In Vitro Fertilization, ISIVF, Copenhagen, Danmark, 27/09/2015.

Effect of environmental pH on sperm kinematic characteristics. / Alipour, Hiva; Dardmeh, Fereshteh; Dissing, Marianne Charlotte; Nielsen, H. Ingolf.

Final programme, 18th World Congress of In Vitro Ferilization, ISIVF 2015, 27-30 September 2015, Copenhagen, Denmark. International Society of In Vitro Fertilization, 2015. s. 22, No. P16.

Publikation: Bidrag til bog/antologi/rapport/konference proceedingKonferenceabstrakt i proceedingForskning

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T1 - Effect of environmental pH on sperm kinematic characteristics

AU - Alipour, Hiva

AU - Dardmeh, Fereshteh

AU - Dissing, Marianne Charlotte

AU - Nielsen, H. Ingolf

PY - 2015/9

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N2 - Semen preparation medium have an important role in assisted reproduction techniques and their composition influences sperm binding and motility.Some studies have assessed the influence of pH on sperm kinetics. However, no study to date has assessed the effect of environmental pH on subtle differences in the details of the sperm movement (kinematics) of human sperm provided by computerized sperm analysis systems. This study was designed to assess the effect of two different media pH levels on kinematic parameters of the human sperm.Samples were prepared using the 40%/80% Pureception (Sage, USA) density gradient and resuspended in customized sperm culture media with pH levels of 7.9 and 8.3 (Origio, Denmark). Kinematic parameters of the sperm in both groups were analyzed using the Sperm Class Analyzer (Microptic S.L., Spain) at 0, 6 and 24 hours following the addition of media.Results of this study illustrated a general insignificant decrease in the ratio of progressively motile and hyperactive sperm after 6 and 24 hours. However a significant difference between the test groups was observed in the curvilinear, straight line and Mean path velocity and Straightness after 6 and 24 hours. Linearity and Wobble showed significant difference after 24 hours.This study demonstrated a difference in the sperm motion pattern and velocity in different environmental pH levels. Based on these findings, further investigations are required to elucidate knowledge about possible effect of marginal pH changes, affecting the sperm motion characteristics at different stages in the dynamic environment of the female reproductive tract.

AB - Semen preparation medium have an important role in assisted reproduction techniques and their composition influences sperm binding and motility.Some studies have assessed the influence of pH on sperm kinetics. However, no study to date has assessed the effect of environmental pH on subtle differences in the details of the sperm movement (kinematics) of human sperm provided by computerized sperm analysis systems. This study was designed to assess the effect of two different media pH levels on kinematic parameters of the human sperm.Samples were prepared using the 40%/80% Pureception (Sage, USA) density gradient and resuspended in customized sperm culture media with pH levels of 7.9 and 8.3 (Origio, Denmark). Kinematic parameters of the sperm in both groups were analyzed using the Sperm Class Analyzer (Microptic S.L., Spain) at 0, 6 and 24 hours following the addition of media.Results of this study illustrated a general insignificant decrease in the ratio of progressively motile and hyperactive sperm after 6 and 24 hours. However a significant difference between the test groups was observed in the curvilinear, straight line and Mean path velocity and Straightness after 6 and 24 hours. Linearity and Wobble showed significant difference after 24 hours.This study demonstrated a difference in the sperm motion pattern and velocity in different environmental pH levels. Based on these findings, further investigations are required to elucidate knowledge about possible effect of marginal pH changes, affecting the sperm motion characteristics at different stages in the dynamic environment of the female reproductive tract.

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SP - 22, No. P16

BT - Final programme, 18th World Congress of In Vitro Ferilization, ISIVF 2015, 27-30 September 2015, Copenhagen, Denmark

PB - International Society of In Vitro Fertilization

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Alipour H, Dardmeh F, Dissing MC, Nielsen HI. Effect of environmental pH on sperm kinematic characteristics. I Final programme, 18th World Congress of In Vitro Ferilization, ISIVF 2015, 27-30 September 2015, Copenhagen, Denmark. International Society of In Vitro Fertilization. 2015. s. 22, No. P16