Effect of short abstinence time on sperm motility parameters

Hiva Alipour, Fereshteh Dardmeh, Gerhard Van Der Horst, G. Manoharan, A. Askeland, H. Ingolf Nielsen

Publikation: Bidrag til bog/antologi/rapport/konference proceedingKonferenceabstrakt i proceedingForskning

Resumé

Semen preparation medium have an important role in assisted reproduction techniques and their composition influences sperm binding and motility.
Some studies have assessed the influence of pH on sperm kinetics. However, no study to date has assessed the effect of environmental pH on subtle differences in the details of the sperm movement (kinematics) of human sperm provided by computerized sperm analysis systems. This study was designed to assess the effect of two different media pH levels on kinematic parameters of the human sperm.
Samples were prepared using the 40%/80% Pureception (Sage, USA) density gradient and resuspended in customized sperm culture media with pH levels of 7.9 and 8.3 (Origio, Denmark). Kinematic parameters of the sperm in both groups were analyzed using the Sperm Class Analyzer (Microptic S.L., Spain) at 0, 6 and 24 hours following the addition of media.
Results of this study illustrated a general insignificant decrease in the ratio of progressively motile and hyperactive sperm after 6 and 24 hours. However a significant difference between the test groups was observed in the curvilinear, straight line and Mean path velocity and Straightness after 6 and 24 hours. Linearity and Wobble showed significant difference after 24 hours.
This study demonstrated a difference in the sperm motion pattern and velocity in different environmental pH levels. Based on these findings, further investigations are required to elucidate knowledge about possible effect of marginal pH changes, affecting the sperm motion characteristics at different stages in the dynamic environment of the female reproductive tract.
OriginalsprogEngelsk
TitelFinal programme, 18th World Congress of In Vitro Ferilization, ISIVF 2015, 27-30 September 2015, Copenhagen, Denmark
ForlagInternational Society of In Vitro Fertilization
Publikationsdato2015
Sider25, No. P36
StatusUdgivet - 2015
BegivenhedWorld Congress of In Vitro Fertilization, ISIVF - Copenhagen, Danmark
Varighed: 27 sep. 201530 sep. 2015
Konferencens nummer: 18

Konference

KonferenceWorld Congress of In Vitro Fertilization, ISIVF
Nummer18
LandDanmark
ByCopenhagen
Periode27/09/201530/09/2015

Fingerprint

Sperm Motility
Spermatozoa
Biomechanical Phenomena
Reproductive Techniques
Denmark
Semen
Spain
Culture Media

Citer dette

Alipour, H., Dardmeh, F., Van Der Horst, G., Manoharan, G., Askeland, A., & Nielsen, H. I. (2015). Effect of short abstinence time on sperm motility parameters. I Final programme, 18th World Congress of In Vitro Ferilization, ISIVF 2015, 27-30 September 2015, Copenhagen, Denmark (s. 25, No. P36). International Society of In Vitro Fertilization.
Alipour, Hiva ; Dardmeh, Fereshteh ; Van Der Horst, Gerhard ; Manoharan, G. ; Askeland, A. ; Nielsen, H. Ingolf. / Effect of short abstinence time on sperm motility parameters. Final programme, 18th World Congress of In Vitro Ferilization, ISIVF 2015, 27-30 September 2015, Copenhagen, Denmark. International Society of In Vitro Fertilization, 2015. s. 25, No. P36
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abstract = "Semen preparation medium have an important role in assisted reproduction techniques and their composition influences sperm binding and motility.Some studies have assessed the influence of pH on sperm kinetics. However, no study to date has assessed the effect of environmental pH on subtle differences in the details of the sperm movement (kinematics) of human sperm provided by computerized sperm analysis systems. This study was designed to assess the effect of two different media pH levels on kinematic parameters of the human sperm.Samples were prepared using the 40{\%}/80{\%} Pureception (Sage, USA) density gradient and resuspended in customized sperm culture media with pH levels of 7.9 and 8.3 (Origio, Denmark). Kinematic parameters of the sperm in both groups were analyzed using the Sperm Class Analyzer (Microptic S.L., Spain) at 0, 6 and 24 hours following the addition of media.Results of this study illustrated a general insignificant decrease in the ratio of progressively motile and hyperactive sperm after 6 and 24 hours. However a significant difference between the test groups was observed in the curvilinear, straight line and Mean path velocity and Straightness after 6 and 24 hours. Linearity and Wobble showed significant difference after 24 hours.This study demonstrated a difference in the sperm motion pattern and velocity in different environmental pH levels. Based on these findings, further investigations are required to elucidate knowledge about possible effect of marginal pH changes, affecting the sperm motion characteristics at different stages in the dynamic environment of the female reproductive tract.",
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Alipour, H, Dardmeh, F, Van Der Horst, G, Manoharan, G, Askeland, A & Nielsen, HI 2015, Effect of short abstinence time on sperm motility parameters. i Final programme, 18th World Congress of In Vitro Ferilization, ISIVF 2015, 27-30 September 2015, Copenhagen, Denmark. International Society of In Vitro Fertilization, s. 25, No. P36, World Congress of In Vitro Fertilization, ISIVF, Copenhagen, Danmark, 27/09/2015.

Effect of short abstinence time on sperm motility parameters. / Alipour, Hiva; Dardmeh, Fereshteh; Van Der Horst, Gerhard; Manoharan, G.; Askeland, A.; Nielsen, H. Ingolf.

Final programme, 18th World Congress of In Vitro Ferilization, ISIVF 2015, 27-30 September 2015, Copenhagen, Denmark. International Society of In Vitro Fertilization, 2015. s. 25, No. P36.

Publikation: Bidrag til bog/antologi/rapport/konference proceedingKonferenceabstrakt i proceedingForskning

TY - ABST

T1 - Effect of short abstinence time on sperm motility parameters

AU - Alipour, Hiva

AU - Dardmeh, Fereshteh

AU - Van Der Horst, Gerhard

AU - Manoharan, G.

AU - Askeland, A.

AU - Nielsen, H. Ingolf

PY - 2015

Y1 - 2015

N2 - Semen preparation medium have an important role in assisted reproduction techniques and their composition influences sperm binding and motility.Some studies have assessed the influence of pH on sperm kinetics. However, no study to date has assessed the effect of environmental pH on subtle differences in the details of the sperm movement (kinematics) of human sperm provided by computerized sperm analysis systems. This study was designed to assess the effect of two different media pH levels on kinematic parameters of the human sperm.Samples were prepared using the 40%/80% Pureception (Sage, USA) density gradient and resuspended in customized sperm culture media with pH levels of 7.9 and 8.3 (Origio, Denmark). Kinematic parameters of the sperm in both groups were analyzed using the Sperm Class Analyzer (Microptic S.L., Spain) at 0, 6 and 24 hours following the addition of media.Results of this study illustrated a general insignificant decrease in the ratio of progressively motile and hyperactive sperm after 6 and 24 hours. However a significant difference between the test groups was observed in the curvilinear, straight line and Mean path velocity and Straightness after 6 and 24 hours. Linearity and Wobble showed significant difference after 24 hours.This study demonstrated a difference in the sperm motion pattern and velocity in different environmental pH levels. Based on these findings, further investigations are required to elucidate knowledge about possible effect of marginal pH changes, affecting the sperm motion characteristics at different stages in the dynamic environment of the female reproductive tract.

AB - Semen preparation medium have an important role in assisted reproduction techniques and their composition influences sperm binding and motility.Some studies have assessed the influence of pH on sperm kinetics. However, no study to date has assessed the effect of environmental pH on subtle differences in the details of the sperm movement (kinematics) of human sperm provided by computerized sperm analysis systems. This study was designed to assess the effect of two different media pH levels on kinematic parameters of the human sperm.Samples were prepared using the 40%/80% Pureception (Sage, USA) density gradient and resuspended in customized sperm culture media with pH levels of 7.9 and 8.3 (Origio, Denmark). Kinematic parameters of the sperm in both groups were analyzed using the Sperm Class Analyzer (Microptic S.L., Spain) at 0, 6 and 24 hours following the addition of media.Results of this study illustrated a general insignificant decrease in the ratio of progressively motile and hyperactive sperm after 6 and 24 hours. However a significant difference between the test groups was observed in the curvilinear, straight line and Mean path velocity and Straightness after 6 and 24 hours. Linearity and Wobble showed significant difference after 24 hours.This study demonstrated a difference in the sperm motion pattern and velocity in different environmental pH levels. Based on these findings, further investigations are required to elucidate knowledge about possible effect of marginal pH changes, affecting the sperm motion characteristics at different stages in the dynamic environment of the female reproductive tract.

M3 - Conference abstract in proceeding

SP - 25, No. P36

BT - Final programme, 18th World Congress of In Vitro Ferilization, ISIVF 2015, 27-30 September 2015, Copenhagen, Denmark

PB - International Society of In Vitro Fertilization

ER -

Alipour H, Dardmeh F, Van Der Horst G, Manoharan G, Askeland A, Nielsen HI. Effect of short abstinence time on sperm motility parameters. I Final programme, 18th World Congress of In Vitro Ferilization, ISIVF 2015, 27-30 September 2015, Copenhagen, Denmark. International Society of In Vitro Fertilization. 2015. s. 25, No. P36