Enhancing Metaproteomics - The value of models and defined environmental microbial systems

Florian-Alexander Herbst, Vanessa Lünsmann, Henrik Kjeldal, Nico Jehmlich, Andreas Tholey, Martin von Bergen, Jeppe Lund Nielsen, Robert L Hettich, Jana Seifert, Per Halkjaer Nielsen

Publikation: Bidrag til tidsskriftReview (oversigtsartikel)peer review

44 Citationer (Scopus)


Metaproteomics - the large-scale characterization of the entire protein complement of environmental microbiota at a given point in time - has provided new features to study complex microbial communities in order to unravel these "black boxes". New technical challenges arose which were not an issue for classical proteome analytics before that could be tackled by the application of different model systems. Here, we review different current and future model systems for metaproteome analysis. Following a short introduction to microbial communities and metaproteomics, we introduce model systems for clinical and biotechnological research questions including acid mine drainage, anaerobic digesters and activated sludge. Model systems are useful to evaluate the challenges encountered within (but not limited to) metaproteomics, including species complexity and coverage, biomass availability or reliable protein extraction. The implementation of model systems can be considered as a step forward to better understand microbial community responses and ecological functions of single member organisms. In the future, improvements are necessary to fully explore complex environmental systems by metaproteomics. This article is protected by copyright. All rights reserved.

Udgave nummer5
Sider (fra-til)783-798
Antal sider16
StatusUdgivet - mar. 2016


Dyk ned i forskningsemnerne om 'Enhancing Metaproteomics - The value of models and defined environmental microbial systems'. Sammen danner de et unikt fingeraftryk.