Microplastics (MPs) were quantified in Danish marine waters of the Kattegat and the southernmost part of Skagerrak bordering to it. Kattegat is a waterbody between Denmark and Sweden that receives inflow from the Baltic Sea and direct urban runoff from the metropolitan area of Copenhagen and Malmö. MPs were measured in 14 continuous transects while steaming between monitoring stations. MP levels tended to be highest close to the Copenhagen-Malmö area, albeit this was more obvious from the abundance of particles rather than mass. The outcome of the measurements allowed a rough MP budget in the Danish Straits region, suggesting that urban waste- and stormwater discharges could not be neglected as potential MP source in these waters. The marine samples were collected by pumping and filtering water over 10 μm steel filters, hereby sampling a total of 19.3 m3. They were prepared and analyzed by FPA-μFTIR imaging, and the scans interpreted to yield MP size, shape, polymer type, and estimated mass. The average concentration was 103 ± 86 items m−3, corresponding to 23.3 ± 28.3 μg m−3 (17–286 items m−3; 0.6–84.1 μg m−3). Most MPs were smaller than 100 μm and fragments dominated the samples. The carbonyl index was assessed for polyolefins, showing that oxidation increased with decreasing MP size, but did not correlate with distance to urban areas. A rough budget of MP in the Danish Straits region suggested that MPs discharged from urban waste- and stormwaters were an import source of MPs.