Extracellular polymeric substances of biofilms: Suffering from an identity crisis

Thomas Seviour, Nicolas Derlon, Morten Simonsen Dueholm, Hans-Curt Flemming, Elisabeth Girbal-Neuhauser, Harald Horn, Staffan Kjelleberg, Mark C.M. van Loosdrecht, Tommaso Lotti, M. Francesca Malpei, Robert Nerenberg, Thomas R. Neu, Etienne Paul, Hanqing Yu, Yuemei Lin

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

2 Citationer (Scopus)

Resumé

Microbial biofilms can be both cause and cure to a range of emerging societal problems including antimicrobial tolerance, water sanitation, water scarcity and pollution. The identities of extracellular polymeric substances (EPS) responsible for the establishment and function of biofilms are poorly understood. The lack of information on the chemical and physical identities of EPS limits the potential to rationally engineer biofilm processes, and impedes progress within the water and wastewater sector towards a circular economy and resource recovery. Here, a multidisciplinary roadmap for addressing this EPS identity crisis is proposed. This involves improved EPS extraction and characterization methodologies, cross-referencing between model biofilms and full-scale biofilm systems, and functional description of isolated EPS with in situ techniques (e.g. microscopy) coupled with genomics, proteomics and glycomics. The current extraction and spectrophotometric characterization methods, often based on the principle not to compromise the integrity of the microbial cells, should be critically assessed, and more comprehensive methods for recovery and characterization of EPS need to be developed.
OriginalsprogEngelsk
TidsskriftWater Research
Vol/bind151
Sider (fra-til)1-7
Antal sider7
ISSN0043-1354
DOI
StatusUdgivet - 15 mar. 2019

Emneord

  • Biofilms
  • Extracellular polymeric substances
  • Function
  • Analysis
  • Biophysics
  • Identity

Citer dette

Seviour, T., Derlon, N., Dueholm, M. S., Flemming, H-C., Girbal-Neuhauser, E., Horn, H., ... Lin, Y. (2019). Extracellular polymeric substances of biofilms: Suffering from an identity crisis. Water Research, 151, 1-7. https://doi.org/10.1016/j.watres.2018.11.020
Seviour, Thomas ; Derlon, Nicolas ; Dueholm, Morten Simonsen ; Flemming, Hans-Curt ; Girbal-Neuhauser, Elisabeth ; Horn, Harald ; Kjelleberg, Staffan ; van Loosdrecht, Mark C.M. ; Lotti, Tommaso ; Malpei, M. Francesca ; Nerenberg, Robert ; Neu, Thomas R. ; Paul, Etienne ; Yu, Hanqing ; Lin, Yuemei. / Extracellular polymeric substances of biofilms: Suffering from an identity crisis. I: Water Research. 2019 ; Bind 151. s. 1-7.
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abstract = "Microbial biofilms can be both cause and cure to a range of emerging societal problems including antimicrobial tolerance, water sanitation, water scarcity and pollution. The identities of extracellular polymeric substances (EPS) responsible for the establishment and function of biofilms are poorly understood. The lack of information on the chemical and physical identities of EPS limits the potential to rationally engineer biofilm processes, and impedes progress within the water and wastewater sector towards a circular economy and resource recovery. Here, a multidisciplinary roadmap for addressing this EPS identity crisis is proposed. This involves improved EPS extraction and characterization methodologies, cross-referencing between model biofilms and full-scale biofilm systems, and functional description of isolated EPS with in situ techniques (e.g. microscopy) coupled with genomics, proteomics and glycomics. The current extraction and spectrophotometric characterization methods, often based on the principle not to compromise the integrity of the microbial cells, should be critically assessed, and more comprehensive methods for recovery and characterization of EPS need to be developed.",
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author = "Thomas Seviour and Nicolas Derlon and Dueholm, {Morten Simonsen} and Hans-Curt Flemming and Elisabeth Girbal-Neuhauser and Harald Horn and Staffan Kjelleberg and {van Loosdrecht}, {Mark C.M.} and Tommaso Lotti and Malpei, {M. Francesca} and Robert Nerenberg and Neu, {Thomas R.} and Etienne Paul and Hanqing Yu and Yuemei Lin",
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Seviour, T, Derlon, N, Dueholm, MS, Flemming, H-C, Girbal-Neuhauser, E, Horn, H, Kjelleberg, S, van Loosdrecht, MCM, Lotti, T, Malpei, MF, Nerenberg, R, Neu, TR, Paul, E, Yu, H & Lin, Y 2019, 'Extracellular polymeric substances of biofilms: Suffering from an identity crisis' Water Research, bind 151, s. 1-7. https://doi.org/10.1016/j.watres.2018.11.020

Extracellular polymeric substances of biofilms: Suffering from an identity crisis. / Seviour, Thomas; Derlon, Nicolas; Dueholm, Morten Simonsen; Flemming, Hans-Curt; Girbal-Neuhauser, Elisabeth; Horn, Harald; Kjelleberg, Staffan; van Loosdrecht, Mark C.M.; Lotti, Tommaso; Malpei, M. Francesca; Nerenberg, Robert; Neu, Thomas R.; Paul, Etienne; Yu, Hanqing; Lin, Yuemei.

I: Water Research, Bind 151, 15.03.2019, s. 1-7.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Extracellular polymeric substances of biofilms: Suffering from an identity crisis

AU - Seviour, Thomas

AU - Derlon, Nicolas

AU - Dueholm, Morten Simonsen

AU - Flemming, Hans-Curt

AU - Girbal-Neuhauser, Elisabeth

AU - Horn, Harald

AU - Kjelleberg, Staffan

AU - van Loosdrecht, Mark C.M.

AU - Lotti, Tommaso

AU - Malpei, M. Francesca

AU - Nerenberg, Robert

AU - Neu, Thomas R.

AU - Paul, Etienne

AU - Yu, Hanqing

AU - Lin, Yuemei

PY - 2019/3/15

Y1 - 2019/3/15

N2 - Microbial biofilms can be both cause and cure to a range of emerging societal problems including antimicrobial tolerance, water sanitation, water scarcity and pollution. The identities of extracellular polymeric substances (EPS) responsible for the establishment and function of biofilms are poorly understood. The lack of information on the chemical and physical identities of EPS limits the potential to rationally engineer biofilm processes, and impedes progress within the water and wastewater sector towards a circular economy and resource recovery. Here, a multidisciplinary roadmap for addressing this EPS identity crisis is proposed. This involves improved EPS extraction and characterization methodologies, cross-referencing between model biofilms and full-scale biofilm systems, and functional description of isolated EPS with in situ techniques (e.g. microscopy) coupled with genomics, proteomics and glycomics. The current extraction and spectrophotometric characterization methods, often based on the principle not to compromise the integrity of the microbial cells, should be critically assessed, and more comprehensive methods for recovery and characterization of EPS need to be developed.

AB - Microbial biofilms can be both cause and cure to a range of emerging societal problems including antimicrobial tolerance, water sanitation, water scarcity and pollution. The identities of extracellular polymeric substances (EPS) responsible for the establishment and function of biofilms are poorly understood. The lack of information on the chemical and physical identities of EPS limits the potential to rationally engineer biofilm processes, and impedes progress within the water and wastewater sector towards a circular economy and resource recovery. Here, a multidisciplinary roadmap for addressing this EPS identity crisis is proposed. This involves improved EPS extraction and characterization methodologies, cross-referencing between model biofilms and full-scale biofilm systems, and functional description of isolated EPS with in situ techniques (e.g. microscopy) coupled with genomics, proteomics and glycomics. The current extraction and spectrophotometric characterization methods, often based on the principle not to compromise the integrity of the microbial cells, should be critically assessed, and more comprehensive methods for recovery and characterization of EPS need to be developed.

KW - Biofilms

KW - Extracellular polymeric substances

KW - Function

KW - Analysis

KW - Biophysics

KW - Identity

U2 - 10.1016/j.watres.2018.11.020

DO - 10.1016/j.watres.2018.11.020

M3 - Journal article

VL - 151

SP - 1

EP - 7

JO - Water Research

JF - Water Research

SN - 0043-1354

ER -