Projekter pr. år
Abstract
In this work, we describe how the application of quantitative proteomics by LC-MS/MS is a highly valuable tool for characterization of protein extracts. In contrast to traditionally employed approaches for protein characterization in food science, proteomics provides protein-level identification and quantification, thereby providing a much deeper level of knowledge about the protein composition in the extracts. Moreover, by applying down-stream bioinformatic analysis of proteomics data, it is possible to predict a probable subcellular localization of the individual proteins. Through comparative analysis with quantitative transcriptomics data, proteomics, in combination with more traditional approaches, can be used to evaluate efficiency and selectivity in the applied extraction method – even in the case of species such as Spinosum, which are quite poorly characterized on the proteome level. Furthermore, we describe how novel bioinformatic algorithms can be applied for prediction of emulsifier and antioxidant peptides embedded in identified proteins. Using this bottom-up discovery approach, we identified and validated the functionality of several novel and potent peptides derived from abundant proteins in Spinosum extracts, thereby increasing their potential applicability and value. Finally, we describe how proteomics data can be used as a supplement to conventional amino acid analysis for evaluating nutritional value of the extracted protein.
Originalsprog | Engelsk |
---|---|
Publikationsdato | 1 okt. 2021 |
Status | Udgivet - 1 okt. 2021 |
Begivenhed | 35th EFFoST International Conference 2021: Healthy Individuals, Resilient Communities, and Global Food Security - SwissTech Convention Center, Lausanne, Schweiz Varighed: 1 nov. 2021 → 4 nov. 2021 http://www.effostconference.com/ |
Konference
Konference | 35th EFFoST International Conference 2021 |
---|---|
Lokation | SwissTech Convention Center |
Land/Område | Schweiz |
By | Lausanne |
Periode | 01/11/2021 → 04/11/2021 |
Internetadresse |
Fingeraftryk
Dyk ned i forskningsemnerne om 'Applying Quantitative Proteomics for Evaluation of Protein Quality, Nutritional Value, and Extraction Methods in Side-Streams of Industrial Carrageenan Production from the Red Seaweed Eucheuma denticulatum (Spinosum)'. Sammen danner de et unikt fingeraftryk.Projekter
- 1 Afsluttet
-
PROVIDE: Protein valorization through informatics, hydrolysis, and separation
Gregersen, S., Overgaard, M. T., Hansen, E. B., Bang-Berthelsen, I., Jacobsen, C., García Moreno, P. J., Marcatili, P. & Yesiltas, B.
01/09/2017 → 30/12/2022
Projekter: Projekt › Forskning
Aktiviteter
- 1 Konferenceoplæg
-
Applying Quantitative Proteomics for Evaluation of Protein Quality, Nutritional Value, and Extraction Methods in Side-Streams of Industrial Carrageenan Production from the Red Seaweed Eucheuma denticulatum (Spinosum
Simon Gregersen (Foredragsholder)
3 nov. 2021Aktivitet: Foredrag og mundtlige bidrag › Konferenceoplæg
-
Emulsifier peptides derived from seaweed, methanotrophic bacteria, and potato proteins identified by quantitative proteomics and bioinformatics
Yesiltas, B., Gregersen Echers, S., Lægsgaard, L., Brinch, M. L., Olsen, T. H., Marcatili, P., Overgaard, M. T., Hansen, E. B., Jacobsen, C. & García-Moreno, P. J., 15 nov. 2021, I: Food Chemistry. 362, 12 s., 130217.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
Åben adgangFil22 Citationer (Scopus)41 Downloads (Pure) -
Quantitative proteomics and bioinformatics in seaweed food protein research: Evaluation of extraction methods, bioactive potential, and nutritional value.
Gregersen Echers, S., Yesiltas, B., García Moreno, P. J., Naseri, A., Holdt, S. L., Jacobsen, C., Hansen, E. B. & Overgaard, M. T., 27 sep. 2021, s. 17. 1 s.Publikation: Konferencebidrag uden forlag/tidsskrift › Konferenceabstrakt til konference › Forskning › peer review
-
AnOxPePred: using deep learning for the prediction of antioxidative properties of peptides
Olsen, T. H., Yesiltas, B., Marin, F. I., Pertseva, M., García-Moreno, P. J., Gregersen Echers, S., Overgaard, M. T., Jacobsen, C., Lund, O., Hansen, E. B. & Marcatili, P., dec. 2020, I: Scientific Reports. 10, 1, 21471.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
Åben adgangFil72 Citationer (Scopus)43 Downloads (Pure)