Fusaoctaxin A, an Example of a Two-Step Mechanism for Non-Ribosomal Peptide Assembly and Maturation in Fungi

Klaus Ringsborg Westphal; Katrine Amalie Hamborg Nielsen; Rasmus Dam Wollenberg; Mathias Bonde Møllerhøj; Simone Bachleitner; Lena Studt; Erik Lysøe; Henriette Giese; Reinhard Wimmer; Jens Laurids Sørensen; Teis Esben Sondergaard

doi:10.3390/toxins11050277

Fusaoctaxin A, an Example of a Two-Step Mechanism for Non-Ribosomal Peptide Assembly and Maturation in Fungi

Klaus Ringsborg Westphal, Katrine Amalie Hamborg Nielsen, Rasmus Dam Wollenberg, Mathias Bonde Møllerhøj, Simone Bachleitner, Lena Studt, Erik Lysøe, Henriette Giese, Reinhard Wimmer, Jens Laurids Sørensen, Teis Esben Sondergaard

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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Abstract

Fungal non-ribosomal peptide synthetase (NRPS) clusters are spread across the chromosomes, where several modifying enzyme-encoding genes typically flank one NRPS. However, a recent study showed that the octapeptide fusaoctaxin A is tandemly synthesized by two NRPSs in Fusarium graminearum. Here, we illuminate parts of the biosynthetic route of fusaoctaxin A, which is cleaved into the tripeptide fusatrixin A and the pentapeptide fusapentaxin A during transport by a cluster-specific ABC transporter with peptidase activity. Further, we deleted the histone H3K27 methyltransferase kmt6, which induced the production of fusaoctaxin A.

Originalsprog	Engelsk
Artikelnummer	277
Tidsskrift	Toxins
Vol/bind	11
Udgave nummer	5
ISSN	2072-6651
DOI	https://doi.org/10.3390/toxins11050277
Status	Udgivet - 16 maj 2019

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10.3390/toxins11050277

Open Access articleForlagets udgivne version, 1,41 MBLicens: CC BY 4.0

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title = "Fusaoctaxin A, an Example of a Two-Step Mechanism for Non-Ribosomal Peptide Assembly and Maturation in Fungi",

abstract = "Fungal non-ribosomal peptide synthetase (NRPS) clusters are spread across the chromosomes, where several modifying enzyme-encoding genes typically flank one NRPS. However, a recent study showed that the octapeptide fusaoctaxin A is tandemly synthesized by two NRPSs in Fusarium graminearum. Here, we illuminate parts of the biosynthetic route of fusaoctaxin A, which is cleaved into the tripeptide fusatrixin A and the pentapeptide fusapentaxin A during transport by a cluster-specific ABC transporter with peptidase activity. Further, we deleted the histone H3K27 methyltransferase kmt6, which induced the production of fusaoctaxin A.",

keywords = "ABC-transporter, Fusarium, Mycotoxins, NRPS, peptidase, secondary metabolites",

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year = "2019",

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doi = "10.3390/toxins11050277",

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T1 - Fusaoctaxin A, an Example of a Two-Step Mechanism for Non-Ribosomal Peptide Assembly and Maturation in Fungi

AU - Westphal, Klaus Ringsborg

AU - Nielsen, Katrine Amalie Hamborg

AU - Wollenberg, Rasmus Dam

AU - Møllerhøj, Mathias Bonde

AU - Bachleitner, Simone

AU - Studt, Lena

AU - Lysøe, Erik

AU - Giese, Henriette

AU - Wimmer, Reinhard

AU - Sørensen, Jens Laurids

AU - Sondergaard, Teis Esben

PY - 2019/5/16

Y1 - 2019/5/16

N2 - Fungal non-ribosomal peptide synthetase (NRPS) clusters are spread across the chromosomes, where several modifying enzyme-encoding genes typically flank one NRPS. However, a recent study showed that the octapeptide fusaoctaxin A is tandemly synthesized by two NRPSs in Fusarium graminearum. Here, we illuminate parts of the biosynthetic route of fusaoctaxin A, which is cleaved into the tripeptide fusatrixin A and the pentapeptide fusapentaxin A during transport by a cluster-specific ABC transporter with peptidase activity. Further, we deleted the histone H3K27 methyltransferase kmt6, which induced the production of fusaoctaxin A.

AB - Fungal non-ribosomal peptide synthetase (NRPS) clusters are spread across the chromosomes, where several modifying enzyme-encoding genes typically flank one NRPS. However, a recent study showed that the octapeptide fusaoctaxin A is tandemly synthesized by two NRPSs in Fusarium graminearum. Here, we illuminate parts of the biosynthetic route of fusaoctaxin A, which is cleaved into the tripeptide fusatrixin A and the pentapeptide fusapentaxin A during transport by a cluster-specific ABC transporter with peptidase activity. Further, we deleted the histone H3K27 methyltransferase kmt6, which induced the production of fusaoctaxin A.

KW - ABC-transporter

KW - Fusarium

KW - Mycotoxins

KW - NRPS

KW - peptidase

KW - secondary metabolites

UR - http://www.scopus.com/inward/record.url?scp=85066282815&partnerID=8YFLogxK

U2 - 10.3390/toxins11050277

DO - 10.3390/toxins11050277

M3 - Journal article

C2 - 31100892

AN - SCOPUS:85066282815

SN - 2072-6651

VL - 11

JO - Toxins

JF - Toxins

IS - 5

M1 - 277

ER -

Fusaoctaxin A, an Example of a Two-Step Mechanism for Non-Ribosomal Peptide Assembly and Maturation in Fungi

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