Genetic variants in microsomal epoxide hydrolase and N-acetyltransferase 2 in susceptibility of IBD in the Danish population

Publikation: Konferencebidrag uden forlag/tidsskriftKonferenceabstrakt til konferenceForskning

Resumé

Introduction. Inflammatory bowel disease (IBD) is characterised by recurrent inflammation of the intestinal mucosa, however the exact mechanism is unknown. Reactive molecules play a central role in the disruption of the mucosa increasing the permeability across the intestinal barrier, which may induce or sustain an immune response. Changes in detoxification of substances that causes epithelial damage may confer susceptibility to IBD. Hence, polymorphic enzymes involved in the detoxification processes may be risk factors of IBD.
Methods. The two biotransformation enzymes microsomal epoxide hydrolase and N-acetyltransferase 2 were genotyped using TaqMan based Real-Time PCR in 388 patients with Crohn's disease (CD), 565 patients with ulcerative colitis (UC) and 796 healthy Danish controls.
Results. No association was found between low microsomal epoxide hydrolase activity or slow N-acetyltransferase 2 acetylator status and IBD. An association between high activity of microsomal epoxide hydrolase and disease diagnosis before age 40 in CD with an OR of 2.2(1.1- 4.2) P=0.02) was found. No other phenotypic associations were found for the two enzymes and IBD, regarding age at onset, disease location, or severity of disease measured either as need for surgery or azathioprine treatment. Smoking was found to be a risk factor of CD (OR=1.8(1.4; 2.3) P<0.001), as opposed to current smoking being a protective factor regarding UC (0.7 (0.5-0.9) P=0.02) which is in agreement with previous findings in other study populations.
Conclusion. Microsomal epoxide hydrolase and N-acetyltransferase 2 appear not to be important in susceptibility of IBD in the Danish population. Nor did we find convincing evidence of associations between the two polymorphic enzymes and phenotypic features in IBD. Smoking was found to be a risk factor of CD and a protective factor regarding UC. Being a complex disease, IBD are most likely dependent on an interaction between genetic and environmental factors.
OriginalsprogEngelsk
Publikationsdato2011
StatusUdgivet - 2011
Udgivet eksterntJa
BegivenhedDSKB - 10. Danske kongres i klinisk biokemi - Aalborg, Danmark
Varighed: 25 maj 201125 maj 2011

Konference

KonferenceDSKB - 10. Danske kongres i klinisk biokemi
LandDanmark
ByAalborg
Periode25/05/201125/05/2011

Citer dette

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title = "Genetic variants in microsomal epoxide hydrolase and N-acetyltransferase 2 in susceptibility of IBD in the Danish population",
abstract = "Introduction. Inflammatory bowel disease (IBD) is characterised by recurrent inflammation of the intestinal mucosa, however the exact mechanism is unknown. Reactive molecules play a central role in the disruption of the mucosa increasing the permeability across the intestinal barrier, which may induce or sustain an immune response. Changes in detoxification of substances that causes epithelial damage may confer susceptibility to IBD. Hence, polymorphic enzymes involved in the detoxification processes may be risk factors of IBD.Methods. The two biotransformation enzymes microsomal epoxide hydrolase and N-acetyltransferase 2 were genotyped using TaqMan based Real-Time PCR in 388 patients with Crohn's disease (CD), 565 patients with ulcerative colitis (UC) and 796 healthy Danish controls.Results. No association was found between low microsomal epoxide hydrolase activity or slow N-acetyltransferase 2 acetylator status and IBD. An association between high activity of microsomal epoxide hydrolase and disease diagnosis before age 40 in CD with an OR of 2.2(1.1- 4.2) P=0.02) was found. No other phenotypic associations were found for the two enzymes and IBD, regarding age at onset, disease location, or severity of disease measured either as need for surgery or azathioprine treatment. Smoking was found to be a risk factor of CD (OR=1.8(1.4; 2.3) P<0.001), as opposed to current smoking being a protective factor regarding UC (0.7 (0.5-0.9) P=0.02) which is in agreement with previous findings in other study populations. Conclusion. Microsomal epoxide hydrolase and N-acetyltransferase 2 appear not to be important in susceptibility of IBD in the Danish population. Nor did we find convincing evidence of associations between the two polymorphic enzymes and phenotypic features in IBD. Smoking was found to be a risk factor of CD and a protective factor regarding UC. Being a complex disease, IBD are most likely dependent on an interaction between genetic and environmental factors.",
author = "Anja Ernst and Vibeke Andersen and Mette {\O}stergaard and Jacobsen, {Bent Ascanius} and Pedersen, {Inge S{\o}kilde} and Drewes, {Asbj{\o}rn Mohr} and Henrik Okkels and H.B. Krarup",
year = "2011",
language = "English",
note = "null ; Conference date: 25-05-2011 Through 25-05-2011",

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Genetic variants in microsomal epoxide hydrolase and N-acetyltransferase 2 in susceptibility of IBD in the Danish population. / Ernst, Anja; Andersen, Vibeke; Østergaard, Mette; Jacobsen, Bent Ascanius; Pedersen, Inge Søkilde; Drewes, Asbjørn Mohr; Okkels, Henrik; Krarup, H.B.

2011. Abstract fra DSKB - 10. Danske kongres i klinisk biokemi, Aalborg, Danmark.

Publikation: Konferencebidrag uden forlag/tidsskriftKonferenceabstrakt til konferenceForskning

TY - ABST

T1 - Genetic variants in microsomal epoxide hydrolase and N-acetyltransferase 2 in susceptibility of IBD in the Danish population

AU - Ernst, Anja

AU - Andersen, Vibeke

AU - Østergaard, Mette

AU - Jacobsen, Bent Ascanius

AU - Pedersen, Inge Søkilde

AU - Drewes, Asbjørn Mohr

AU - Okkels, Henrik

AU - Krarup, H.B.

PY - 2011

Y1 - 2011

N2 - Introduction. Inflammatory bowel disease (IBD) is characterised by recurrent inflammation of the intestinal mucosa, however the exact mechanism is unknown. Reactive molecules play a central role in the disruption of the mucosa increasing the permeability across the intestinal barrier, which may induce or sustain an immune response. Changes in detoxification of substances that causes epithelial damage may confer susceptibility to IBD. Hence, polymorphic enzymes involved in the detoxification processes may be risk factors of IBD.Methods. The two biotransformation enzymes microsomal epoxide hydrolase and N-acetyltransferase 2 were genotyped using TaqMan based Real-Time PCR in 388 patients with Crohn's disease (CD), 565 patients with ulcerative colitis (UC) and 796 healthy Danish controls.Results. No association was found between low microsomal epoxide hydrolase activity or slow N-acetyltransferase 2 acetylator status and IBD. An association between high activity of microsomal epoxide hydrolase and disease diagnosis before age 40 in CD with an OR of 2.2(1.1- 4.2) P=0.02) was found. No other phenotypic associations were found for the two enzymes and IBD, regarding age at onset, disease location, or severity of disease measured either as need for surgery or azathioprine treatment. Smoking was found to be a risk factor of CD (OR=1.8(1.4; 2.3) P<0.001), as opposed to current smoking being a protective factor regarding UC (0.7 (0.5-0.9) P=0.02) which is in agreement with previous findings in other study populations. Conclusion. Microsomal epoxide hydrolase and N-acetyltransferase 2 appear not to be important in susceptibility of IBD in the Danish population. Nor did we find convincing evidence of associations between the two polymorphic enzymes and phenotypic features in IBD. Smoking was found to be a risk factor of CD and a protective factor regarding UC. Being a complex disease, IBD are most likely dependent on an interaction between genetic and environmental factors.

AB - Introduction. Inflammatory bowel disease (IBD) is characterised by recurrent inflammation of the intestinal mucosa, however the exact mechanism is unknown. Reactive molecules play a central role in the disruption of the mucosa increasing the permeability across the intestinal barrier, which may induce or sustain an immune response. Changes in detoxification of substances that causes epithelial damage may confer susceptibility to IBD. Hence, polymorphic enzymes involved in the detoxification processes may be risk factors of IBD.Methods. The two biotransformation enzymes microsomal epoxide hydrolase and N-acetyltransferase 2 were genotyped using TaqMan based Real-Time PCR in 388 patients with Crohn's disease (CD), 565 patients with ulcerative colitis (UC) and 796 healthy Danish controls.Results. No association was found between low microsomal epoxide hydrolase activity or slow N-acetyltransferase 2 acetylator status and IBD. An association between high activity of microsomal epoxide hydrolase and disease diagnosis before age 40 in CD with an OR of 2.2(1.1- 4.2) P=0.02) was found. No other phenotypic associations were found for the two enzymes and IBD, regarding age at onset, disease location, or severity of disease measured either as need for surgery or azathioprine treatment. Smoking was found to be a risk factor of CD (OR=1.8(1.4; 2.3) P<0.001), as opposed to current smoking being a protective factor regarding UC (0.7 (0.5-0.9) P=0.02) which is in agreement with previous findings in other study populations. Conclusion. Microsomal epoxide hydrolase and N-acetyltransferase 2 appear not to be important in susceptibility of IBD in the Danish population. Nor did we find convincing evidence of associations between the two polymorphic enzymes and phenotypic features in IBD. Smoking was found to be a risk factor of CD and a protective factor regarding UC. Being a complex disease, IBD are most likely dependent on an interaction between genetic and environmental factors.

M3 - Conference abstract for conference

ER -

Ernst A, Andersen V, Østergaard M, Jacobsen BA, Pedersen IS, Drewes AM et al. Genetic variants in microsomal epoxide hydrolase and N-acetyltransferase 2 in susceptibility of IBD in the Danish population. 2011. Abstract fra DSKB - 10. Danske kongres i klinisk biokemi, Aalborg, Danmark.