@article{a79954314a8d4133bcf06c777f111218,
title = "Genome-centric view of carbon processing in thawing permafrost",
abstract = "As global temperatures rise, large amounts of carbon sequestered in permafrost are becoming available for microbial degradation. Accurate prediction of carbon gas emissions from thawing permafrost is limited by our understanding of these microbial communities. Here we use metagenomic sequencing of 214 samples from a permafrost thaw gradient to recover 1,529 metagenome-assembled genomes, including many from phyla with poor genomic representation. These genomes reflect the diversity of this complex ecosystem, with genus-level representatives for more than sixty per cent of the community. Meta-omic analysis revealed key populations involved in the degradation of organic matter, including bacteria whose genomes encode a previously undescribed fungal pathway for xylose degradation. Microbial and geochemical data highlight lineages that correlate with the production of greenhouse gases and indicate novel syntrophic relationships. Our findings link changing biogeochemistry to specific microbial lineages involved in carbon processing, and provide key information for predicting the effects of climate change on permafrost systems.",
author = "Woodcroft, {Ben J.} and Singleton, {Caitlin M.} and Boyd, {Joel A.} and Evans, {Paul N.} and Emerson, {Joanne B.} and Zayed, {Ahmed A.F.} and Hoelzle, {Robert D.} and Lamberton, {Timothy O.} and McCalley, {Carmody K.} and Hodgkins, {Suzanne B.} and Wilson, {Rachel M.} and Purvine, {Samuel O.} and Nicora, {Carrie D.} and Changsheng Li and Steve Frolking and Chanton, {Jeffrey P.} and Crill, {Patrick M.} and Saleska, {Scott R.} and Rich, {Virginia I.} and Tyson, {Gene W.}",
note = "Funding Information: Acknowledgements This study was funded by the Genomic Science Program of the United States Department of Energy (DOE) Office of Biological and Environmental Research (BER), grants DE-SC0004632, DE-SC0010580 and DE-SC0016440. B.J.W. and P.N.E. are supported by Australian Research Council Discovery Early Career Research Awards #DE160100248 and #DE170100428, respectively. C.M.S. and J.A.B. are supported by Australian Government Research Training Program (RTP) Scholarships, and G.W.T. is supported by Australian Research Council Future Fellowship FT170100070. A portion of the research was performed using Environmental Molecular Sciences Laboratory (EMSL), a DOE Office of Science User Facility, and a portion was performed under the Facilities Integrating Collaborations for User Science (FICUS) initiative with resources at both the DOE Joint Genome Institute and EMSL. Both facilities are sponsored by the Office of BER and operated under contracts DE-AC02-05CH11231 (JGI) and DE-AC05-76RL01830 (EMSL). We thank the IsoGenie 1 and 2 Project Teams and the 2010–2012 field teams for sample collection, particularly T. Logan, as well as the Abisko Scientific Research Station for sampling infrastructure and support. We thank P. Hugenholtz, D. Parks, S. Robbins, B. Kemish, M. Chuvochina, S. Low and M. Butler for helpful discussion and infrastructure support. Publisher Copyright: {\textcopyright} 2018, Macmillan Publishers Ltd., part of Springer Nature.",
year = "2018",
month = aug,
day = "2",
doi = "10.1038/s41586-018-0338-1",
language = "English",
volume = "560",
pages = "49--54",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "7716",
}