Resumé

The biosynthetic pathway of the Fusarium pigments bikaverin (PKS16) and fusarubins (PKS3) are reconstructed utilizing a plasmid-based expression system and heterologously expressed in Saccharomyces cerevisiae. This system allows for a quick and easy assembly of biosynthetic pathways for pathway-metabolite linkage while simultaneously obtaining the target metabolite without other secondary metabolites, due to the simplistic secondary metabolism of the heterologous expression host. The application of heterologous expression allows for exploitation of the beneficial function of the target metabolite, without the concerns of malignant byproducts expressed by Fusarium sp. This allows for beneficial applications of the end-product such as novel drug discovery and expression in large scale industrial fermentation. The two biosynthetic pathways reconstructed in this study, functions as proof-of-concept that entire PKS-pathways can be expressed in S. cerevisiae and a visible phenotypic change occurs when translation is induced.
OriginalsprogEngelsk
Publikationsdato3 apr. 2019
StatusUdgivet - 3 apr. 2019
BegivenhedNordic-Baltic Fusarium seminar - Esbjerg, Danmark
Varighed: 3 apr. 20194 jul. 2019

Seminar

SeminarNordic-Baltic Fusarium seminar
LandDanmark
ByEsbjerg
Periode03/04/201904/07/2019

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Biosynthetic Pathways
Fusarium
Saccharomyces cerevisiae
Secondary Metabolism
Drug Discovery
Fermentation
Plasmids

Citer dette

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title = "Heterologous expression of biosynthetic pathways from Fusarium",
abstract = "The biosynthetic pathway of the Fusarium pigments bikaverin (PKS16) and fusarubins (PKS3) are reconstructed utilizing a plasmid-based expression system and heterologously expressed in Saccharomyces cerevisiae. This system allows for a quick and easy assembly of biosynthetic pathways for pathway-metabolite linkage while simultaneously obtaining the target metabolite without other secondary metabolites, due to the simplistic secondary metabolism of the heterologous expression host. The application of heterologous expression allows for exploitation of the beneficial function of the target metabolite, without the concerns of malignant byproducts expressed by Fusarium sp. This allows for beneficial applications of the end-product such as novel drug discovery and expression in large scale industrial fermentation. The two biosynthetic pathways reconstructed in this study, functions as proof-of-concept that entire PKS-pathways can be expressed in S. cerevisiae and a visible phenotypic change occurs when translation is induced.",
author = "Pedersen, {Tobias Bruun} and Nielsen, {Mikkel Rank} and S{\o}rensen, {Jens Laurids} and Kristensen, {Sebastian Birkedal}",
year = "2019",
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Pedersen, TB, Nielsen, MR, Sørensen, JL & Kristensen, SB 2019, 'Heterologous expression of biosynthetic pathways from Fusarium' Nordic-Baltic Fusarium seminar, Esbjerg, Danmark, 03/04/2019 - 04/07/2019, .

Heterologous expression of biosynthetic pathways from Fusarium. / Pedersen, Tobias Bruun; Nielsen, Mikkel Rank; Sørensen, Jens Laurids; Kristensen, Sebastian Birkedal.

2019. Poster præsenteret på Nordic-Baltic Fusarium seminar, Esbjerg, Danmark.

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskning

TY - CONF

T1 - Heterologous expression of biosynthetic pathways from Fusarium

AU - Pedersen, Tobias Bruun

AU - Nielsen, Mikkel Rank

AU - Sørensen, Jens Laurids

AU - Kristensen, Sebastian Birkedal

PY - 2019/4/3

Y1 - 2019/4/3

N2 - The biosynthetic pathway of the Fusarium pigments bikaverin (PKS16) and fusarubins (PKS3) are reconstructed utilizing a plasmid-based expression system and heterologously expressed in Saccharomyces cerevisiae. This system allows for a quick and easy assembly of biosynthetic pathways for pathway-metabolite linkage while simultaneously obtaining the target metabolite without other secondary metabolites, due to the simplistic secondary metabolism of the heterologous expression host. The application of heterologous expression allows for exploitation of the beneficial function of the target metabolite, without the concerns of malignant byproducts expressed by Fusarium sp. This allows for beneficial applications of the end-product such as novel drug discovery and expression in large scale industrial fermentation. The two biosynthetic pathways reconstructed in this study, functions as proof-of-concept that entire PKS-pathways can be expressed in S. cerevisiae and a visible phenotypic change occurs when translation is induced.

AB - The biosynthetic pathway of the Fusarium pigments bikaverin (PKS16) and fusarubins (PKS3) are reconstructed utilizing a plasmid-based expression system and heterologously expressed in Saccharomyces cerevisiae. This system allows for a quick and easy assembly of biosynthetic pathways for pathway-metabolite linkage while simultaneously obtaining the target metabolite without other secondary metabolites, due to the simplistic secondary metabolism of the heterologous expression host. The application of heterologous expression allows for exploitation of the beneficial function of the target metabolite, without the concerns of malignant byproducts expressed by Fusarium sp. This allows for beneficial applications of the end-product such as novel drug discovery and expression in large scale industrial fermentation. The two biosynthetic pathways reconstructed in this study, functions as proof-of-concept that entire PKS-pathways can be expressed in S. cerevisiae and a visible phenotypic change occurs when translation is induced.

M3 - Poster

ER -

Pedersen TB, Nielsen MR, Sørensen JL, Kristensen SB. Heterologous expression of biosynthetic pathways from Fusarium. 2019. Poster præsenteret på Nordic-Baltic Fusarium seminar, Esbjerg, Danmark.