Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium
solani, it was possible to produce the core scaold compound of bostrycoidin, a red aza-anthraquinone
pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated
recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the
inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production
of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1–4 days,
where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L.
TidsskriftInternational Journal of Molecular Sciences (Online)
Udgave nummer21
StatusUdgivet - 14 okt. 2020

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