TY - JOUR
T1 - Heterologous expression of the core genes in the complex fusarubin gene cluster of Fusarium Solani
AU - Pedersen, Tobias Bruun
AU - Nielsen, Mikkel Rank
AU - Kristensen, Sebastian Birkedal
AU - Spedtsberg, Eva Mia Lang
AU - Yasmine, Wafaa
AU - Matthiesen, Rikke
AU - Kaniki, Samba Evelyne Kabemba
AU - Sørensen, Trine
AU - Petersen, Celine
AU - Muff, Jens
AU - Sondergaard, Teis Esben
AU - Nielsen, Kåre Lehmann
AU - Wimmer, Reinhard
AU - Sørensen, Jens Laurids
N1 - Funding Information:
Funding: This study was supported by grants from The Danish Research Council, Technology, and Production (Grant No. 7017-00167) and the Novo Nordisk Foundation (NNF18OC0034952).
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/10/2
Y1 - 2020/10/2
N2 - Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1-4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L.
AB - Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1-4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L.
KW - Bostrycoidin
KW - Fungi
KW - Fusarium
KW - Heterologous expression
KW - Pigments
KW - Polyketides
KW - Yeast
UR - http://www.scopus.com/inward/record.url?scp=85092926943&partnerID=8YFLogxK
U2 - 10.3390/ijms21207601
DO - 10.3390/ijms21207601
M3 - Journal article
C2 - 33066643
AN - SCOPUS:85092926943
VL - 21
SP - 1
EP - 10
JO - International Journal of Molecular Sciences (Online)
JF - International Journal of Molecular Sciences (Online)
SN - 1661-6596
IS - 20
M1 - 7601
ER -
