Heterologous expression of the core genes in the complex fusarubin gene cluster of Fusarium Solani

Tobias Bruun Pedersen, Mikkel Rank Nielsen, Sebastian Birkedal Kristensen, Eva Mia Lang Spedtsberg, Wafaa Yasmine, Rikke Matthiesen, Samba Evelyne Kabemba Kaniki, Trine Sørensen, Celine Petersen, Jens Muff, Teis Esben Sondergaard, Kåre Lehmann Nielsen, Reinhard Wimmer, Jens Laurids Sørensen*

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Abstrakt

Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1-4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L.

OriginalsprogEngelsk
Artikelnummer7601
TidsskriftInternational Journal of Molecular Sciences
Vol/bind21
Udgave nummer20
Sider (fra-til)1-10
Antal sider10
ISSN1661-6596
DOI
StatusUdgivet - 2 okt. 2020

Bibliografisk note

Funding Information:
Funding: This study was supported by grants from The Danish Research Council, Technology, and Production (Grant No. 7017-00167) and the Novo Nordisk Foundation (NNF18OC0034952).

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

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