Heterologous expression of the core genes in the complex fusarubin gene cluster of Fusarium Solani

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Abstrakt

Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1-4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L.

OriginalsprogEngelsk
Artikelnummer7601
TidsskriftInternational Journal of Molecular Sciences
Vol/bind21
Udgave nummer20
Sider (fra-til)1-10
Antal sider10
ISSN1661-6596
DOI
StatusUdgivet - 2 okt. 2020

Bibliografisk note

Funding Information:
Funding: This study was supported by grants from The Danish Research Council, Technology, and Production (Grant No. 7017-00167) and the Novo Nordisk Foundation (NNF18OC0034952).

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

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